For Immunolocalization
Sorry
No idea.
Not my field.
Regards
Bernardo Rapoport
I am trying to perform a siRNA on cGAS gene on HACAT cells and also HCT116 as control. I am using a smartpool purchased from Dharmacon (ON-TARGETplus siRNA MB21D1) following the manufacturer's...
12 April 2023 9,511 0 View
How is it possible to obtain a more or less homogeneous solution containing cutin?
21 March 2023 5,212 0 View
Dear colleague we are doing an update of the eucalypt surface all over the World. Could you please send me any avaliable information on this topic in your specific country or elsewhere? Please...
13 October 2021 9,034 9 View
Is there a way to aggregate an existing relationship between two sub-nodes to higher level nodes? For example, let us say you have "code 1" with an underlying "sub-code 1" and "code 2" with an...
07 March 2021 4,260 3 View
I have already test c-myc Ab sc40 (9E10) and sc789 (A14) from santa cruz and also ab32072 (Y69) from abcam with 40µg of mouse liver extract but no one works!
09 April 2019 3,691 2 View
I'm looking for an expert in Mysida whoc an help me in identifying a species (probably a Gastrosaccus) but that presents two spines on the carapace, I didn't find in the bibliography I have and...
03 March 2019 2,015 4 View
Is this cell line commercially available? Thank you for your help.
08 August 2018 5,786 0 View
Dear all, Recently the technology in SDD disk have been developed by increasing the read-write velocities and the "lifetime" of writes such hundreds of TB. Even so, the available memory is very...
06 April 2018 7,050 8 View
This is a rare opportunity to investigate the fire-invasion continuum by observing the conditions before, during and after fire. Likewise, these experiments may provide original data for other...
26 March 2018 4,372 4 View
Tools such as PhP metrics are limited to Object Oriented code and dependent on fixed structures. I'm searching for tools to analyse students non OO code. I've already tested: "Sonarqube",...
10 January 2018 8,298 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View