A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
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Questions related to Immunoassay
Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...
08 August 2019 2,745 5 View
My Immunoassay is giving higher analyte concentrations for the same sample before plasma separation vs after plasma separation. For instance, I run my assay with whole blood, and I get a reading,...
11 July 2019 7,565 3 View
We have already detected IL-6 and some other cytokines are increased in our mice bone marrow supernatant by multiplex immunoassays. Now we want to know which cells secrete IL-6. Many cells can...
21 June 2019 6,912 4 View
06 June 2019 3,202 5 View
We do not have a system for oxygen plasma, corona discharge or ozone in our lab. We wanted to create reactive surface for our immunoassay on PDMS, such that it can bind to APTES. We have been...
29 April 2019 9,780 3 View
Can we use same amount of whole blood in place of serum/plasma for quantitation of hormones, while making sure RBCs are not lysed, in a non-chromatographic immunoassay? Would RBCs by themselves...
22 April 2019 5,793 3 View
04 April 2019 1,310 3 View
04 April 2019 3,472 3 View
To be specific the substrates are from GE lifesciences , FF80 HP and FF120 HP
28 March 2019 4,404 2 View
03 March 2019 3,089 2 View
Our ISurBlock works like BSA to block unwanted protein adsorption in immunoassay, but returns a lower signal. How can we tell if it's blocking NSB? That is, is it interfering with specific...
03 March 2019 3,677 2 View
I carry out ELISA experiments, in which a log/log curve fit is required in order to calculate concentration from O.D. I have used the drc package previously to fit a 4PL model, but this kit...
26 February 2019 8,737 7 View
Dear all, I have a slightly odd question regarding immunogold silver enhancement. I am aiming to look at 2 targets in the same tissue section, but I need a way to differentiate them using metal...
22 February 2019 7,911 2 View
Hi, I am trying to do a lateral flow immunoassay with antibodies (1mg / mL) immobilized in nitrocellulose (pore of 0.45 um). My line of antibodies is 4 mm x 1 mm, however I can not obtain a...
02 February 2019 8,734 3 View
Process flow for assigning Calibrators & Control values in any diagnostics assays e.g. Biochecmistry, Immunoassays (ELISA) /multi standard mode. What would be the major difference in the...
01 January 2019 8,190 0 View
Hi everybody. I am interested to know what is different between: electrochemiluminescence (ECL) method and chemiluminescent immunoassay (CLIA) method ? TNX... Kambbiz
17 December 2018 6,529 3 View
12 December 2018 1,881 4 View
RLU values are changing daily for the same kit and control in the CLIA assay on fully automated analyser. Can any one help in this issue regarding the significance of variatin of RLU values and...
12 December 2018 3,924 3 View
I'm doing some statistical analysis where the point is to compare two different types of samples (CSF and Plasma) that where measured for the same protein in an immunoassay. The controls don't...
12 December 2018 10,081 4 View
My kit recommended generating a four parameter logistic (4-PL) curve-fit for my standards; however, I can't find any tutorials for how to do that on GraphPad. I did find a tutorial for generating...
08 December 2018 6,089 7 View
03 December 2018 8,811 3 View
I am gonna optimise an incubation buffer for proximity extension assay (PEA), and I am wondering if I am missing anything vital in the buffer. This buffer is mainly for cell lysate detecting...
30 November 2018 788 1 View
11 November 2018 8,107 1 View
We have reconstituted human TSH hormone (Sigma), that we use in our immunoassay system. How long can we keep the same at 4 deg before any sort of loss in activity is observed? We do multiple test...
10 October 2018 4,568 0 View