A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
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Questions related to Immunoassay
I want to analyze immuno-dominant antigens of parasites. I extract crude protein and want to check it's immunogenicity using an immunoassay. And I want to find the size of each antigen? is there...
10 October 2014 9,407 3 View
Hi! All, I am doing ELIspot for the first time and am having problems getting spots even with concanavalin stimulated spleen cells. I am seeing spots with the naked eye but when I look under a...
10 October 2014 1,882 12 View
The cortisol RIA we have been using for years (Siemens DPC Coat-a-count) to measure plasma levels in non-human primates is being discontinued. Does anyone have a recommendation for another...
23 September 2014 2,744 4 View
09 September 2014 5,286 5 View
I am working on developing a luminex sandwich immunoassay and discovered that the literature (luminex xMAPCookBook) recommends carrying out a coupling efficiency test. I used the same antibody...
22 August 2014 7,986 3 View
08 August 2014 2,991 3 View
I'm trying to conjugate an antibody to silver nanoparticles (20 nm diameter, citrate capped AgNPs). The conjugation protocol essentially involves centrifuging AgNP stock, resuspending at the same...
08 August 2014 5,449 2 View
Most of the protocols i've found on papers about attach proteins in glass has some extreme variations about the process... My goal is to attach proteins labelled with FITC in glass plates and...
06 June 2014 2,976 1 View
We recently purchased macrophage like cell RAW 264.7 from European collection of cell cultures. The cells have been thawed according to original handling procedure for frozen cells. We have now...
05 May 2014 1,815 6 View
I'm quantifying proteins on western blots, using chemiluminescent ECL primer and a CCD-based imager (Carestream) to visualize the bands. I can use exposure times of seconds to minutes. The shorter...
18 April 2014 5,568 2 View
Has anyone tried fixing cells in an MSD plate? We normally use 80% acetone but I don't know if this will interfere with MSD detection. I would also like to use the same antigen in a sandwich...
04 April 2014 7,161 2 View
I am doing research on autophagy with bacteria. According to our lab protocol, first we leave the cells (RAW264.7) infected with bacteria for an hour at MOI=10, then wash out the cells in order to...
04 April 2014 4,113 2 View
02 April 2014 10,019 2 View
I found in an immunization scheme that a mouse had large thyroid. This scheme was with T4-BSA as immunogen.
11 February 2014 7,143 2 View
02 February 2014 1,421 2 View
I am trying to optimize ADCC for HIV infected cells. I am labeling my targets with CFSE and any other membrane or nuclear dye. My effectors are unlabeled. The problem is the second dye, no matter...
16 January 2014 7,846 3 View
I was wondering if it is reasonable to use half the amount of beads & sample when running a novex luminex kit? I'd sure like to get 2x the use out of this expensive kit. If so, what settings would...
01 January 2014 9,945 1 View
We are starting a project on peanut allergy. I'm looking for an appropriate commercial source of the peanut allergen extract as well as purified Ara h proteins, especially h1 and h2.
12 December 2013 10,101 7 View
I am trying to measure arginase activity in serum samples from cancer patients and in cancer cell lysates. I am using the colorimetric assay according to Corraliza et al (in brief: I take 22,5 ul...
12 December 2013 4,891 2 View
We have met several cases of different HBsAg results by different equipment. How to confirm which one is true?
12 December 2013 9,221 4 View
I'm performing IHC on mouse tissue. My primary antibodies are rat anti-mouse antibody. I would like to know which is the best secondary anti-rat HRP conjugated DAB detection system ki. There are...
15 November 2013 454 5 View
We are looking for non-competitive antibody for a new immunoassay to monitor MAO-B Inhibition that should also enable us to detect inactive enzyme-inhibitor complexes.
11 November 2013 4,408 0 View
I have tried a histopaque isolation, but I get a lot of RBCs in my final pellet. When I later put these cells on a 7 day CFSE assay with PHA 0.5ug/ml as a positive control, I find two problems....
11 November 2013 242 2 View
I am currently developing an antigen-down assay for Troponin I. I have already optimized the surface deposition of Troponin I, but am looking for ways to increase the kinetic binding parameters of...
11 November 2013 6,699 2 View