40nm Citrate Gold Nanoparticle Thiol Ligand Exchange, Without Aggregation?

I wish to exchange citrate on my 40nm gold particles for a thiol (prochimia.com/products/product/104), however, I always seem to aggregate the gold particles: the initial red colour goes blue, or...

11 December 2017 1,070 5 View

Can I buy a commercial TMB solution for immunoblotting onto nitrocellulose that is free of peroxide?

I wish to find a commercial source of TMB solution formulated to deposit blue TMB precipitate onto nitrocellulose, but has no added peroxide. Can you help?

07 August 2017 9,543 1 View

How do I use VEDA to do quick PED of isotopomers?

I have used VEDA to perform PED for my molecule. The vibrational analysis was done in Gaussian 09W. How do I get Gaussian to provide files for VEDA to do PED using the same internal coordinates...

01 February 2016 8,623 0 View

Anharmonic Raman Intensities in Gaussian 09W Revision A.02?

I wish to compute anharmonic Raman intensities in Gaussian 09W Revision A.02. I have computed the anharmonic IR frequencies which can be found in the output file. How do I set up the input file to...

01 February 2016 1,101 1 View

How do I remove non-specific binding from my lateral flow assay?

My lateral flow assay uses a gold particle linked capture and secondary surface bound antibody; both mouse Mabs. The sample is human blood and I'm looking for a disease protein marker. How can I...

09 October 2015 8,630 6 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

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01 March 2021 3,905 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View