A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
1,376 views 358 posts
Questions related to Immunoassay
I need a to perform an immunoassay of TNF-alpha on human serum. I am looking for advise on brands that can offer a good (the best if possible) balance price/quality/simplicity. If you could share...
07 December 2015 9,732 2 View
Hi everyone! We're hoping to run an ELISPOT using splenocytes animals that have been cured of tumors with an antibody drug. We are fine with isolating the splenocytes, but are worried about the...
14 November 2015 7,725 3 View
I am trying to develop a lateral flow immuno-assay for Neisseria lactamica and i want to visualise and determine the movement of the bacterial cells in the sample and conjugate membranes by...
11 November 2015 5,772 0 View
I'm very beginner in the development of competitive lateral flow immunochromatographic assay for small molecule detection and facing with the the problem of false negative result . the result of...
11 November 2015 5,612 2 View
11 November 2015 5,403 3 View
I'm developing multiplexed bead based indirect immunoassay for four target cytokines and developing sandwich immunoassay for each. I have measured the performance of each individually and I am...
10 October 2015 1,359 1 View
Hello. I am interested to hear advices on how to measure HAT activity from immunoprecipitated samples. After preparing a cell lysate, I have been using a HAT activity assay kit with...
09 September 2015 6,816 3 View
I am not seeing any signal after ELISPOT assay and wondering if I can re-probe.
08 August 2015 6,946 3 View
Can I use a spectrophotometer in the place of an absorbance plate reader, to measure plasma adiponectin using R&D immunoassay kits?
08 August 2015 353 3 View
According to abcam: If an antibody is working in IP, this is a good indication that it will work in RIP. Please suggest me. Thanks in advance
08 August 2015 5,844 2 View
Antibody for glutamatergic neurons bodyfor immunohistochemistry in frozen PFA fixed cuts. Regions of interest: Retrosplenial cortex and hippocampus
08 August 2015 1,871 2 View
I want to extract miRNAs from serum or plasma, there are lots of kits from different companies, eg Qiagen, Exiqon or ABI. Which one is the best ? Have anybody compared those reagents? Any suggestions?
08 August 2015 8,288 2 View
Three Maxisorb 96-well plates from the same sleeve produced identical patterns of high absorbancies in wells that should not have had such signals. All 3 plates were double sandwich ELISAs. One...
07 July 2015 3,033 2 View
What blocking buffers can I use for a Lateral Flow Immunoassay? (NCM Blocking, after spraying)
07 July 2015 6,772 2 View
I want to measure C-peptide concentration to check the insulin secretion by MIN6 by means of ELISA, but don't want to buy an ELISA kit. The problem is that I can't find biotin conjugated C-peptide...
07 July 2015 416 7 View
I have a plate made of ABS (something like 96-well ELISA plate) with square-shaped wells. I intend to use it to do dot immunoassay. ABS is hydrophobic so buffer solutions do not spread over the...
07 July 2015 8,233 7 View
I have had success with the LM609 clone on human tissue,but it doesn't react with rat. Currently working with some rabbit polyclonals and would like some input on others' experiences. Thanks,
07 July 2015 8,545 1 View
Been doing beta-hexosaminidase release assay but the results hasn't been promising. Firstly, there's no colour development in the supernatant and secondly the release has been below 10% for all...
07 July 2015 3,867 6 View
I would like to induce quiesence in a cell line and trace recovery. Is there an effective way to do this?
07 July 2015 6,468 5 View
There is a plethora of publications on lateral flow immunoassays and SERS detection, and most papers hint at point-of-care capabilities of this technology. However, you apparently cannot buy a...
07 July 2015 9,718 0 View
I have never used it and would like to know if others have been satisfied with it, especially regarding its accuracy and precision.
06 June 2015 9,461 0 View
In 1998 we published an article showing a new simple statistical method for determining endpoint titer in immunoassays. Please see abstract below. Can anyone can suggest a useful method when no...
06 June 2015 9,990 0 View
Is there any published papers that support the above question?
06 June 2015 6,330 1 View
I'm doing an ELISA to determine the amount of corticosterone in rat serum and it looks like my research assistant made some pipetting errors and allowed a little plasma to be collected in a few of...
06 June 2015 4,292 3 View