Why are there very few health devices based on electrochemical sensors or other simple assay technologies?

Electrochemical sensors have an inherent advantage for being simple, cheap (glucometers), especially for nucleic acid assays. Yet there are very few health devices in the market for point of care...

11 December 2019 8,547 3 View

How can we stabilize purified humanThyroid Stimulating Hormone (hTSH) in buffer, without the use of albumin or other large molecules?

We use purified hTSH (in PBS), but have issue with stability of the sample. Since the purified hormone is highly unstable the activity reduces drastically at room temperature. We had added BSA to...

06 July 2019 8,619 4 View

What are some good methods (other than Plasma/Ozone/UV treament) for generation of reactive molecules before using APTES for coating in microchannels?

We do not have a system for oxygen plasma, corona discharge or ozone in our lab. We wanted to create reactive surface for our immunoassay on PDMS, such that it can bind to APTES. We have been...

03 April 2019 3,379 3 View

How likely are antibodies in buffer to get degraded by protease contamination due to casual handling?

I know it could be a little naive question. Wanted to gather views and experiences on likelihood of stock solutions of antibodies, in PBS (with sodium azide) likely to suffer degradation due to...

02 March 2019 5,073 7 View

What can be the reasons that you get a lower reading for samples than control for an immunoassay?

We are performing a microfluidics based immunoassay reaction, with a modified system. We have been getting lower readings for our spiked samples, compared to control samples. For example, if...

31 December 2018 6,351 3 View

How long can you store a reconstituted TSH sample in a refrigerator?

We have reconstituted human TSH hormone (Sigma), that we use in our immunoassay system. How long can we keep the same at 4 deg before any sort of loss in activity is observed? We do multiple test...

09 October 2018 4,450 0 View

What can be the reason for decreased ELISA reading of reconstituted TSH hormone?

We recently got TSH hormone from Sigma. On the first day, we ran our assay, and the results were fine. On the second day, the activity has somehow reduced by 90%. How is this possible? We...

07 August 2018 348 2 View

What can be done to increase efficiency of reconstitution of desiccated microparticles in a microchannel?

We are trying to desiccate BSA coated polystyrene microparticles (2-3 um diameter) in a plastic microchannel blocked with BSA/Casein + Tween 20. When we are trying to reconstitute the particles,...

07 August 2018 8,638 0 View

What would be the best coating buffer for covalent coating of TSH antibody (IEF Range: 5.8-6.9) on a functionalised PMMA surface?

We are optimizing the assay parameters for our TSH immunoassay. We had been using a 7.4 pH PBS buffer for antibody coating so far, but now want to evaluate if changing the pH of the coating buffer...

06 July 2018 3,644 2 View

Best method to desiccate reagents and polymeric microparticles in a microchannel?

We wish to desiccate protein coated polystyrene microparticles with some other proteins inside a microchannel. We tried doing so using normal air drying (non-sterile air, from a blower). But when...

05 June 2018 3,806 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View