A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
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Questions related to Immunoassay
Hello Everybody, does anybody of you have experience with ELISA kits by Cloud-Clone Corp.? I'm particularly interested in SEA108Mu (OPG-ELISA) and SEA855Mu (RANKL-ELISA). Thanks a lot.
06 June 2015 8,117 2 View
I would like to assess the reliability of different immunoassay methods (nephelometry, EMIT, immune diffusion etc) for C-reactive protein. I felt correlation studies using human blood samples...
06 June 2015 2,565 3 View
Hi. I have some question regarding detection. If I am using HRP antibody and I will use hydrogen peroxide as substrate. What my stop solution, how much time I need to wait for the reaction, and...
06 June 2015 3,641 3 View
Hello, I want to do western blotting with Jurkat cells. Up to now I did western blotting with solid tumors by using RIPA 0.5-1.0 buffer and 15 minutes of centrifugation at 14.000g. I used the...
06 June 2015 8,238 3 View
I would like to know if the cortisol RIA kit for human (serum & saliva) can be used for detection of the cortisol level in bovine serum?
06 June 2015 891 3 View
I want to perform Hardy Weinberg Equilibrium test for killer immunoglobulin like receptors (KIRs). I think using this test for genes like KIRs is not true, because sum of the allele frequencies is...
21 May 2015 4,088 3 View
I am searching for analytical standards for beta-estradiol and testosterone to use in spike-recovery experiments to validate ELISA kits. What form of standard is best to use? I see that most are...
05 May 2015 6,369 7 View
We are developing the T3 streptavidin based assay. Currently we tried different T3 mab. These mab gives good results when coated directly on ELISA plate. But after biotinylation, mabs behaves...
05 May 2015 2,870 8 View
Is it possible to induce active EAE with MOG 35-55 using a TLR ligand (CPG?) as the adjuvant instead of CFA?
05 May 2015 9,103 0 View
Dear researcher. I research the immunoassay by using CA125 antibody-antigen in microfluidic system. And my detection method is the fluorescence detection by using the FITC. So I used the product :...
05 May 2015 4,297 3 View
05 May 2015 9,161 3 View
Hello, I'm trying to get this technique to work on K ion channel subunits in murine 4% PFA fixed spinal cord slices that have been treated with Na Citrate for 30mins at 70 degrees and blocked for...
05 May 2015 5,535 5 View
I need to storage my plasma samples at -80 °C until I'll collect all of them. I'll measure T3, T4, cortisol and so on by ELISA, but i need to know the stability of those analites from plasma at -80°C
05 May 2015 5,654 7 View
Hello immunology experts, I'm interested in studying cross-presentation of antigens into the MHC-class I pathway, and there is sufficient evidence that this can be driven through various...
05 May 2015 6,064 5 View
In a protocol datasheet, of a particular cytokine ELISA It is suggested to start reagent diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. What...
05 May 2015 9,877 2 View
I need help with making precoated ELISA plates, especially preserving. I never did this before, but it will save me a lot of time. What I found with google search is: incubate with capture...
04 April 2015 5,592 6 View
Many novel immunoassays methods employed functional (antibodies-conjugated) nanoparticles as novel probe to detect analytes. My question is how to determine the number of antibodies conjugated on...
04 April 2015 8,189 8 View
This analysis was meant to verify the presence of my recombinant protein that is transiently expressed in Nicotiana benthamiana. The transgene consists of a chloroplast targeting peptide (CTP) and...
04 April 2015 8,550 3 View
In regard to label-free assays developed to measured interactions between some target molecule and a surface, I often see the Langmuir isotherm/1:1 binding model applied to systems without...
04 April 2015 8,099 5 View
A postdoc position is available in my lab at the University of Kansas, starting in summer 2015. The project is focused on developing new microfluidic immunoassays for ultrasensitive detection of...
04 April 2015 9,495 2 View
Hello everyone. I want to confirm protein-protein interaction using the PLA assay in human cd4-t-cells. I would be very happy on any experience regarding how good it works, choice of antibody and...
16 March 2015 9,463 3 View
What is the best way to test whether an ECL kit for western blot detection is working or not?
03 March 2015 2,765 4 View
03 March 2015 6,315 3 View
In the past we have used Diasorin's ACTH IRMA for assays in human and rhesus plasma. This kit has been discontinued, and I am looking for a replacement. I have tried MP Biomedicals RIA and MD...
03 March 2015 4,321 4 View