A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody... | Contact experts in Immunoassay to get answers
2,299 views 358 posts
Questions related to Immunoassay
I am faced with an experiment that involves a large number of mice that require elispot and flow cytometry to be done on the same day., n=4 x12 groups, stimulated with 10 different peptides. will...
03 March 2015 4,250 3 View
ELISA or immunoassay blocking buffer
03 March 2015 8,936 4 View
I am confused with LOD and cutoff value. Can anyone explain it? If possible, please share the different methods for finding the above mentioned value?
03 March 2015 2,383 4 View
The method for measuring testosterone is being switched from immunoassay to liquid chromatography tandem mass spec and I am establishing a new reference range for both sexes as well as the free...
02 February 2015 6,382 3 View
Ortho-Clinical Vitros ECi tests sometimes give borderline result. so i need the reason for this
02 February 2015 1,155 1 View
The FACS machine at our core facility has a 670 LP (long pass) filter to detect PerCP and PerCP-Cy5.5. Yet, unlike the band pass (BP) filters these seem to accept a large chunk of the emission...
02 February 2015 1,935 6 View
I had used Angiotensin II EIA Kit cat.no. RAB0010 from SIGMA but the results are confusing... I cannot understand why standart curve must be built by 1,000 pg/ml, 100 pg/ml, 10 pg/ml, 1 pg/ml, 0.1...
30 January 2015 7,917 4 View
We are interested in measuring total ApoB in triglyceride-rich lipoprotein fractions from plasma from our human subjects by ELISA. Would like to hear any recommendations for the ELISA kits...
22 January 2015 2,287 3 View
hello, I used ovalbumin as antigen to immunize mouse, I want find out whether the antibody affinity maturation or not, there is a good way? Thank you.
07 January 2015 1,428 5 View
01 January 2015 8,083 5 View
We used AKTA prime machine to purify the serum and now we need to choose which one of the techniques above mentioned are most suitable for further use. To purify and measure the quantity of Ab in...
01 January 2015 9,733 6 View
The total reaction volume suggested by the provided protocol is 1.8 mL. However, 0.1-0.2 mL are enough to read the absorbance of the chromogenic reaction. Is there any good reason for not scaling...
01 January 2015 8,532 1 View
01 January 2015 8,067 4 View
What are the causes of false positive and false negative of amphetamine in urine by semi quantitative immunoassay? I am using Roche analyzer for presumptive screening, what are the chances of...
01 January 2015 2,905 3 View
Hello. I need an advise. Is there any method for the detection of CA 15-3 and PSA except immunoassay? Thanks
01 January 2015 9,628 4 View
We are receiving a shipment of blood for screening potential new monkeys in our facility, and due to weather complications they will sit for longer than we had hoped before they are processed. It...
01 January 2015 478 1 View
Hi everyone, I recently stained cells for CD4 and B220 from axillary and mesenterial lymph nodes. After FACS analysis there was a very defined population for CD4- B220+. Whats the type of this...
12 December 2014 8,561 0 View
Genomic DNA is digested with a restriction enzyme, end labeled with biotin-dNTP with TdT, and then immunoprecipitated with Protein G Dynabeads coated with anti-biotin antibody.
12 December 2014 10,073 15 View
Hello everyone Can I add streptavidin (with PE, FITC, APC etc.) to my biotin-conjugated antibody before incubation with my cells? I want use this antibody to FACS analysis. Thanks for your answers.
12 December 2014 3,370 5 View
05 December 2014 8,284 14 View
I am wondering how one can avoid saturation of loading controls while detecting low abundance protein of interest. I usually load 15-20microgram/lane of nuclear fraction from tissue homogenate to...
11 November 2014 1,105 5 View
When used the immunoassay method, several metabolites are able to interfere with salivary cortisol determination especially in sample from neonates, therapy replacement, oral prednisolone use...
11 November 2014 3,007 3 View
I'm trying to stain for a 6xHis-tagged protein which accumulated in skin tissue in mice. I want to use mouse polyclonal antibody to 6XHis tag (Sigma), conjugated to HRP (one step procedure) and...
11 November 2014 6,287 7 View
I am trying Matrigel invasion assay for myeloma cell lines. I am using 8 microns membrane and coating the matrigel at 1: 3 conc. But am not getting results. I am using U266 cell line which is...
17 October 2014 10,054 3 View