We have already detected IL-6 and some other cytokines are increased in our mice bone marrow supernatant by multiplex immunoassays. Now we want to know which cells secrete IL-6. Many cells can secrete IL-6. We can sort out different types of bone marrow cells and detect the cytokine mRNA. But I am wondering that if we do a IHC or Immunofluorescence at first, will it be helpful in identify which cells have higher possibility through the morphology of positive cells or their localization in bone marrow?
Hi, Tong
Please read the article that is being sent to you.
Hope to be useful
I'd say fluorescence is your best bet, Tong. IF stainings are tricky and often end up in artifacts due to high background stemming from poorly characterized antibodies. Personal opinion, of course... But stemming from years of (often frustrating) experience. Good luck in your endeavor - whatever choice you make!
IF for double labelling plus nuclear labelling and ISH on parallel sections,
Good luck
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