How to quantify the charge on dyes like Rhodamine and Tartrazine?

I am working on flow of cationic (Methylene blue Cat. no.: M9140 and Rhodamine B: R6626) and anionic (Tartrazine: T0388 and Amaranth: A1016) dyes on paper substrates. All the dyes are from Sigma...

09 October 2019 1,916 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Colorimteric and enzymatic method ?

First of all, thank you for your consideration about this question. I've been working on development of in-vitro diagnostic medical device as research engineer since 2013. Especially, my major is...

14 January 2021 3,885 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

Is there any method to convert color on nitrocellulose into digits (numbers)?

This is to design a protocol in which color obtained on nitrocellulose membrane can be converted or processed through any method to digits (numbers) to compare with different state of particular...

06 January 2021 9,309 1 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

What is optimal buffer for saliva immunoglobulins testing with lateral flow immunoassay?

Hi to everyone! Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type? I think that i need a buffer for LFIA becouse saliva too...

16 November 2020 1,520 3 View

Can primary antibodies bind to analyte coupled magnetic beads directly if the coupling was unsuccessful ?

I am working with multiplex immunoassay. As I am new into multiplexing I am facing some problem. I have coupled Protein X to carboxylated magnetic beads (BioRad) using EDC-NHS. Now, while...

08 November 2020 9,911 7 View