What Proteome Information should hunt after from core seminal plasma proteome?

Dear all I have mapped the proteome of human seminal plasma. Furthermore, I have compare it with the plasma proteome. I want to know what information I can extract from my core seminal plasma...

09 October 2018 3,766 2 View

Analytical or Preparative 2DE gel for image analysis?

Hi Everyone We are running 2DE in our lab. I am in doubt what is best for running image analysis: It is best to run a analytical or preparative 2DE gel for image analysis between control or...

08 September 2018 3,460 0 View

FDA approved metabolite identification by HPLC or LCMS?

Hi Everybody I am looking for a paper or the list of FDa approved metabolite identified by HPLC or LCMS? Can you help me out? Merci, Kambiz

05 June 2018 8,692 7 View

What is the number of all possible metabolic reactions?

Hi Everybody. I am looking for a paper or database about the number of all possible metabolic reaction in different organisms e.g. human or E.coli! Merci

31 December 2016 8,447 2 View

How many genome have been sequenced so far?

Hi Everybody. I am looking for documentation about how many genome have been sequenced so far. Additionally, how many gene are found from these organism? Merci, Kambiz

31 December 2016 9,085 0 View

What tense to use to write a good review paper?

Hi Everybody I want to write a good review based on my results and the literature. However, I do not know which tense I should use? Is it dependent on journal I have choose? Merci, Kambiz

09 October 2016 722 6 View

Plasma proteome vs seminal plasma proteome. How can I do it in a smart way?

Hi Everybody. I have collected more than 2000 proteins from human seminal plasma. I want to compare it to the human plasma proteome. Is there any smart software out there which can help me...

05 June 2016 3,406 2 View

How to define Systems Proteomics?

Hi Fellows. I was wondering how to define Systems Proteomics? Does anybody have a good suggestion. I have attache those paper I could find regarding this approach... Merci, Kambiz

05 June 2016 7,056 5 View

Human Taurine ELISA kit for urine study ...

Hi Everybody. I want to buy Human Taurine ELISA kit for urinary study. I was wondering have anybody used it? What should I take care about when I am going to use it? Merci, Kambiz

04 May 2016 1,903 2 View

How many 2DE gels run for clinical proteomics trial?

Hej Everybody. I am looking for to get answer how many 2DE gels I have to run for a pilot study in clinical proteomics? Should I pooled the sample and run three 2DE-gels or just run 3 good...

04 May 2016 6,630 4 View

Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Has annyone try to reuse EveryBlot Blocking Buffer (Bio-rad) for fluorescence western blotting?

Hello to every one, Our lab has recently switched our western blot technique from chemiluminescence to fluorescence. Therefore, we have to optimize the blocking buffer. We have successfully try...

05 February 2021 7,886 1 View

How to use Image Lab to take an image for the bands and the ladder in the same image merged together in Western Blot?

Hello everyone. I was trying to use Image Lab software to take an image of both the ladder and the samples merged together in one image to see the position of the bands according to the ladder MW,...

04 February 2021 9,996 2 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Colorimteric and enzymatic method ?

First of all, thank you for your consideration about this question. I've been working on development of in-vitro diagnostic medical device as research engineer since 2013. Especially, my major is...

14 January 2021 3,885 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View