ECL versus CLIA? - FAQS.TIPS

More Kambiz Gilany's questions See All

What Proteome Information should hunt after from core seminal plasma proteome?

Dear all I have mapped the proteome of human seminal plasma. Furthermore, I have compare it with the plasma proteome. I want to know what information I can extract from my core seminal plasma...

09 October 2018 3,870 2 View

Analytical or Preparative 2DE gel for image analysis?

Hi Everyone We are running 2DE in our lab. I am in doubt what is best for running image analysis: It is best to run a analytical or preparative 2DE gel for image analysis between control or...

08 September 2018 3,594 0 View

FDA approved metabolite identification by HPLC or LCMS?

Hi Everybody I am looking for a paper or the list of FDa approved metabolite identified by HPLC or LCMS? Can you help me out? Merci, Kambiz

05 June 2018 8,800 7 View

What is the number of all possible metabolic reactions?

Hi Everybody. I am looking for a paper or database about the number of all possible metabolic reaction in different organisms e.g. human or E.coli! Merci

31 December 2016 8,549 2 View

How many genome have been sequenced so far?

Hi Everybody. I am looking for documentation about how many genome have been sequenced so far. Additionally, how many gene are found from these organism? Merci, Kambiz

31 December 2016 9,203 0 View

What tense to use to write a good review paper?

Hi Everybody I want to write a good review based on my results and the literature. However, I do not know which tense I should use? Is it dependent on journal I have choose? Merci, Kambiz

09 October 2016 809 6 View

How to define Systems Proteomics?

Hi Fellows. I was wondering how to define Systems Proteomics? Does anybody have a good suggestion. I have attache those paper I could find regarding this approach... Merci, Kambiz

05 June 2016 7,162 5 View

Plasma proteome vs seminal plasma proteome. How can I do it in a smart way?

Hi Everybody. I have collected more than 2000 proteins from human seminal plasma. I want to compare it to the human plasma proteome. Is there any smart software out there which can help me...

05 June 2016 3,519 2 View

Human Taurine ELISA kit for urine study ...

Hi Everybody. I want to buy Human Taurine ELISA kit for urinary study. I was wondering have anybody used it? What should I take care about when I am going to use it? Merci, Kambiz

04 May 2016 2,000 2 View

How many 2DE gels run for clinical proteomics trial?

Hej Everybody. I am looking for to get answer how many 2DE gels I have to run for a pilot study in clinical proteomics? Should I pooled the sample and run three 2DE-gels or just run 3 good...

04 May 2016 6,760 4 View

What are the typical gels used in EMSA experiments (non-radioactive) to detect shift in bands of protein & RNA complexes?

I would like to hear if anyone here has had prior or present experience with Lightshift Chemiluminescent RNA EMSA kit from Thermo Scientific? What are the gels you have used to detect the labelled...

23 June 2024 7,463 1 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

How could I analyze the results of caspase-glo 3/7 assay?

I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...

02 June 2024 7,833 2 View

What is best stabilizing buffer for conjogation of quantum dots to antibodies?

We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...

25 May 2024 7,663 3 View

Substitutes for Dako anti-HBcAg rabbit polyclonal?

I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...

28 April 2024 3,860 1 View

Medical lab quality/risk professionals - do you use medical goals? Will you share?

Since 1999, the recommended source of allowable error limits or Analytical Performance Standards has been to set limits based on medical need in specific clinical settings. I have been doing this...

09 April 2024 7,424 2 View

How do results of Bland-Altman plots and regression analysis contribute to our understanding of the comparability between serum tubes and LIH tubes?

In the journal, "THE TRANSITION FROM GEL SEPARATORY SERUM TUBES TO LITHIUM HEPARIN GEL TUBES IN THE CLINICAL LABORATORY" by Oğuzhan Zengi, how do the results of Bland-Altman plots and regression...

09 April 2024 7,249 1 View

Aside from the diagnostic efficacy of the various immunoassays for HDV, do these tests vary in risks or health hazard for the medical technologist?

Pertaining to the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Pan et. al (2023), they discussed the...

09 April 2024 4,044 1 View

What are the factors that make lithium heparinized plasma (LIH) tubes more viable for use in clinical laboratories?

The journal "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" aims to assess the viability of replacing serum with plasma samples in...

08 April 2024 3,341 3 View

Western blot trouble shooting (detection problem)?

I have two questions: 1. After transfer, the marker confirmed that the transfer had proceeded well. However, I would like to know why only one protein band appears in the high kDa region when...

29 March 2024 1,830 0 View

Jean Rene Grezes

Hi,

It seems that you are not familiar with searching with Google/Wikipedia.

Here :

https://en.wikipedia.org/wiki/Electrochemiluminescence

see the references too

and the same for:

https://en.wikipedia.org/wiki/Chemiluminescence

See please the introduction of the Symposium Ser. 18(1985) (see copy)

Contact please the companies like Amersham and others for papers or booklets

Good luck

JRG

Kambiz Gilany

I can not find this documentation your are writing in this blog. Additionally, if I was expert in ECL or CLIA, I would not asked...

TNX

Please tell me if you have read the 2 Wikipedia articles about ECL and CLIA. You can see the difference in technical aspects. If you contact the companies selling apparatus for ECL and CLIA, you can earn , free of charge, a lot of papers.

Good Luck.

TNX... I will do that...