06 June 2019 5 3K Report

We have already detected IL-6 and some other cytokines are increased in our mice bone marrow supernatant by multiplex immunoassays. Now we want to know which cells secrete IL-6. Many cells can secrete IL-6. We can sort out different types of bone marrow cells and detect the cytokine mRNA. But I am wondering that if we do a IHC or Immunofluorescence at first, will it be helpful in identify which cells have higher possibility through the morphology of positive cells or their localization in bone marrow?

Similar questions and discussions