I am gonna optimise an incubation buffer for proximity extension assay (PEA), and I am wondering if I am missing anything vital in the buffer.

This buffer is mainly for cell lysate detecting proteins, but should be made for plasma/other samples as well.

My start recipe looks like this:

1x PBS, 0.1% BSA, 0.01% NaN3, single stranded sperm DNA, (0.2% Tween? MgCl2?)

What incubation buffers do you use in your assays, and whats in them?

Thank you!