ECL versus CLIA? - FAQS.TIPS

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Energy consumption control with an approach of Iranian architecture?

I am looking for a partner who can help in reducing energy consumption in the field of Iranian architecture.

04 January 2024 2,195 3 View

Does aggregation induced quenching (AIQ) impact on absorption of CQDs?

Hi Usually, AIQ has an effect on the emission spectrum. What about absorption? Does aggregation-induced quenching (AIQ) impact on the absorption of CQDs?

19 August 2023 3,481 1 View

Have anybody a list of secondary amino acids in order to see antioxidant capacity?

Hey everybody. I have done amino acids analysis by LCMSMS. I am looking for a list of secondary amino acids in order to see antioxidant capacity in these molecules. anyone have a such list???? TNX

12 June 2023 5,845 3 View

Can Raman spectra nbe used as quantification in metabolic fingerprinting?

Dear all, I am using metabolic fingerprinting for my clinical studies. I am in doubt that I can use the Raman spectra for quantification? Can I use it semi-quantify? Can you provide me with...

11 April 2022 7,753 2 View

How to calculate amino acids concentrations when I am using isotope labeled compounds as internal standards in LCMSMS?

Dear All. I have separated 38 amino acids on HILIC column couple to QQQ followed by using 10 isotope-labeled amino acids as internal standard. However, I am not sure which formula to use to...

28 December 2021 2,543 4 View

Aggregation-induced quenching (AIQ)?

Hi I want to do Research about Carbon QDs. papers have talked about aggregation-induced quenching (AIQ) and I can not get detailed information about AIQ from the papers. could you please give...

28 December 2020 4,663 0 View

Call for reviewers & manuscripts?

Dear Professors and Researchers Peace be upon all of you. It is a great pleasure to announce that The Egyptian Journal of Community Medicine is no available at this link. All publications are...

29 December 2019 6,052 1 View

How to determine the sub x in a structure such as CuInSexS2−x QDs?

hello every one I want to synthesis the CuInSexS2-x QDs and as I am elementary in chemistry, I want to know that how to determine the sub x in such structure in practice (mean in the synthesis of...

24 November 2019 2,271 1 View

Does glycidol react with OH or carboxylic group in citric acid? which one has higher tendency to react with epoxide ring?

As you know, citric acid has three carboxylic acids with pka: 3.13, 4.76, and 6.4 as well as a hydroxyl group with pka 11.6 to 14.5 (depending on the reference). On the other hand, OH has a potent...

10 November 2019 10,096 2 View

How to chose semiconductor material for synthesis of colloidal QD?

Hello I want to know, how to chose appropriate semiconductor material for QD synthesis? it is base on bandgap? or something else? how scientists know that the I-III-VI elements (for example) are...

07 November 2019 828 2 View

What are the typical gels used in EMSA experiments (non-radioactive) to detect shift in bands of protein & RNA complexes?

I would like to hear if anyone here has had prior or present experience with Lightshift Chemiluminescent RNA EMSA kit from Thermo Scientific? What are the gels you have used to detect the labelled...

23 June 2024 7,463 1 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

How could I analyze the results of caspase-glo 3/7 assay?

I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...

02 June 2024 7,833 2 View

What is best stabilizing buffer for conjogation of quantum dots to antibodies?

We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...

25 May 2024 7,663 3 View

Substitutes for Dako anti-HBcAg rabbit polyclonal?

I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...

28 April 2024 3,860 1 View

Medical lab quality/risk professionals - do you use medical goals? Will you share?

Since 1999, the recommended source of allowable error limits or Analytical Performance Standards has been to set limits based on medical need in specific clinical settings. I have been doing this...

09 April 2024 7,424 2 View

How do results of Bland-Altman plots and regression analysis contribute to our understanding of the comparability between serum tubes and LIH tubes?

In the journal, "THE TRANSITION FROM GEL SEPARATORY SERUM TUBES TO LITHIUM HEPARIN GEL TUBES IN THE CLINICAL LABORATORY" by Oğuzhan Zengi, how do the results of Bland-Altman plots and regression...

09 April 2024 7,249 1 View

Aside from the diagnostic efficacy of the various immunoassays for HDV, do these tests vary in risks or health hazard for the medical technologist?

Pertaining to the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Pan et. al (2023), they discussed the...

09 April 2024 4,044 1 View

What are the factors that make lithium heparinized plasma (LIH) tubes more viable for use in clinical laboratories?

The journal "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" aims to assess the viability of replacing serum with plasma samples in...

08 April 2024 3,341 3 View

Western blot trouble shooting (detection problem)?

I have two questions: 1. After transfer, the marker confirmed that the transfer had proceeded well. However, I would like to know why only one protein band appears in the high kDa region when...

29 March 2024 1,830 0 View

Jean Rene Grezes

Hi,

It seems that you are not familiar with searching with Google/Wikipedia.

Here :

https://en.wikipedia.org/wiki/Electrochemiluminescence

see the references too

and the same for:

https://en.wikipedia.org/wiki/Chemiluminescence

See please the introduction of the Symposium Ser. 18(1985) (see copy)

Contact please the companies like Amersham and others for papers or booklets

Good luck

JRG

Kambiz Gilany

I can not find this documentation your are writing in this blog. Additionally, if I was expert in ECL or CLIA, I would not asked...

TNX

Please tell me if you have read the 2 Wikipedia articles about ECL and CLIA. You can see the difference in technical aspects. If you contact the companies selling apparatus for ECL and CLIA, you can earn , free of charge, a lot of papers.

Good Luck.