Hi everyone!

We would like to perform this protein synthesis in my lab in order to then purify it for hybridomas development in mouse. We have found a plasmid that suits our requirements (AmpR, IPTG inducible, His tag and TEV domain) and also an E. coli strain (SHuttle T7) that is a priori capable of fold it correctly. However, we are not really sure if this integrin will be correctly processed in this system because it has a single-pass transmembrane domain, so maybe it will interfere with the correct processing in the cells... Does anyone know if our design is correct and if this protein will be correctly expressed in our bacterial system?

Thank you in advance!