Hi,
I am planning to do EM analysis on exosomes derived from neural progenitor cells. I have quantified the amount of exosomes that I got from my prep by BCA assay and it gave me a concentration of 597.6 ug/ml in 30 ul, which would make a total of 17.9 ug. I am wondering now, if this amount might be enough for EM analysis? I am mainly interested in morphology of exosomes.
Thanks a lot!
I have run single particle samples as low as 1-10 ug/ml, although some literature has a 70 ug/ml suggested concentration. The standard negative stain recommendations are in the 100-300 ug/ml range. I would try your concentration on glow discharged grids and make sure you blot the sample onto the grid after absorption. Also run a sample free buffer as a control. If you sample contains phosphate, use filtered 1% phosphotungstic acid, pH 7.2 as a negative stain. Good luck
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