Check your EM manager about the protocol for diffraction mode, and it could be microscope dependent.

I would like to suggest to read this paper entitled: Electron Diffraction Using Transmission Electron Microscopy [J. Res. Natl. Inst. Stand. Technol. 106, 997–1012 (2001)]

https://www.researchgate.net/publication/258735610_Electron_Diffraction_of_Protein_3D_Nanocrystals

Article Electron Diffraction of Protein 3D Nanocrystals

Dear Vladimir, thanks for citing our publication.

Dear Cristina,

there are some differences and similarities compared to X-ray crystallography.

1. Your samples will need to be about 100-200nm thin and have to be vitrified to withstand the vacuum of an electron microscope. Vitrification is broadly used in Cryo-EM.

2. You will need a sensitive detector capable of collecting diffraction patterns at extremely low dose. We used a Timepix Detector, now marketed by Amsterdam Scientific Instruments.

3. You can collect rotation data like you would with X-Ray Crystallography and use X-ray programs to solve the structure.

Please also look at our Acta paper for more info on sample prep and data collection and analysis.

Good luck! feel free to e-mail me if you have any further questions.

Cheers,

Igor

Thank you so much for all the answers! I will carefully look at them!

Sorry for the delayed answered! I did not see it till now!

Hi! I didn't mention that I would like to do it for amyloids! Is it still possible? Thanks!