How to prepare bacterial culture for Scanning Electron Microscope (SEM) study?

More Muhammad Saleem Kalhoro's questions See All

Can we judge the quality of scientific journal by its impact factor alone?

What are other good indicators of quality journal apart from high impact factor

10 November 2018 7,971 24 View

How to save Excel graph as image with 300 DPI?

I tried both MS paint and MS word, then saved as image but DPI is 96, conversion from 96 to 300 DPI through software dose not improve image quality, I am looking for direct method to save Excel...

08 September 2017 1,900 43 View

How to prepare 20 mM sodium acetate buffer (pH 4.6)?

How to prepare 20 mM sodium acetate buffer (pH 4.6)

06 July 2017 8,425 4 View

How to prepare 100 ml HCL-KCL 10 mM buffer?

How to prepare 100 ml HCL-KCL 10 mM buffer, pH 4.6Will there be a change in pH after autoclave?

04 May 2017 9,292 2 View

How to add Tertiary Vertical axis in Line Graph?

I want to add tertiary vertical axis in line graph as seen in attached picture

04 May 2017 4,595 4 View

Does Journal Impact Factor Really Matters when getting PhD?

31 December 2016 7,904 6 View

Can I get Gene accession number by submitting single strand 16S rDNA sequence to NCBI or Double strand 16S rDNA sequence is required for this purpose?

Can I get Gene accession number by submitting single strand 16S rDNA sequence of bacteria to NCBI or Double strand 16S rDNA sequence is required for this purpose?

31 December 2016 10,137 6 View

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree?

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree

31 December 2016 5,022 9 View

How long prepared PBS 7.0 (Phosphate Buffered Saline) can be stored?

PBS 7.2 (Himedia) adjusted to pH 7.0 stored at 4-8 °C in refrigerator

09 October 2016 4,343 3 View

How to select 1% bacterial culture for inoculation purpose?

Read 6 answers by scientists with 6 recommendations from their colleagues to the question asked by Muhammad Saleem Kalhoro on May 5, 2016

04 May 2016 8,387 7 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Alwin David Popular answer

for detail:

file:///C:/Users/ADMIN/Downloads/infocus%20%2310%20June%202008%20Conventional%20Scanning%20Electron%20Microscopy%20of%20Bacteria.pdf

http://pubmedcentralcanada.ca/pmcc/articles/PMC379936/pdf/applmicro00035-0122.pdf

Alwin David

Jai Ghosh

The best way would be to do gold deposition using vapors under vacuum or you may use the splutter method also. It depends on what facilities you have at your end.