Please guide: Why my PCR lacks reproducibilty?

whenever i buy new primers (100pM) they work fine at first dilution (10pM) as well as first 3-4 polymerase chain reactions. But when i use same or new dilutions (10pM) after 15- 20 days at the...

20 July 2020 5,371 16 View

What can be possible reason when there is no reproducibilty in PCR which ones functioned?

When i run gradient PCR, it works well for 2-3 days, After that there is no amplification neither in same or other gradient ranges nor at temperature specified by first run. Is this issue with...

17 March 2020 1,643 2 View

What is the concept of mass collaboration ?

collaboration meaning

13 June 2019 4,233 3 View

What is the equivalent conditions for strongly \pi regular rings?

hello im looking for a equivalent conditions for strpngly \pi regular rings. a ring R is said to be strongly \pi regular if for each x in R there exist a y in R such that x^n = (x ^(n+1))y. do...

31 December 2018 9,118 3 View

Large eddy simulation in film cooling?

I am recently working on a large eddy simulation in film cooling process. I would like to ask you to guidance me in this field. Due to the high number of mesh and small time steps in large eddy...

25 November 2018 9,318 4 View

What are the considerations for Wind Tunnel Testing of a 152 mm chord length airfoil model in test section of 300mm x 100mm x 50mm test section?

I am working on an airfoil model of 152mm chord length x 46mm span. I have made a wind tunnel test section of 300mm x 100mm x 50mm.. It is a low subsonic WT upto 35 m/s. I want to compare the WT...

02 August 2018 4,322 3 View

Dose 'endomorphism ring of power series rings' has a special properties ?

hi i want to know about endomorphism ring of power series rings. if anyone know about that or road a paper about that please share it with me. thank you

28 April 2018 5,480 2 View

What are the analytical possibilities of a full-SEM (with LVs) method that are not available in EFA?

I have used a full SEM- (containing latent variables) for analysis. My Questions (See my simple example to better understand the questions.): Example: Consider three LV's A (with indicators a1,...

29 July 2017 832 5 View

I performing the test of moderation for Sex (Male- Female) in AMOS ?

29 January 2017 8,925 3 View

Do you know a good bioinformatic online site to predict the targets of chemical compounds?

I am looking for a reliable web site which I can give chemical name or molecular formula of compounds and software can identify the target proteins.

18 July 2016 3,297 6 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View