Hi every one, I our Skin Lab research, we focus in Pemphigus Vulgaris diseases, specific the association of ST18 gene with PV. We read the article "Etesami, Ifa, et al. "The association between ST18 gene polymorphism and severe pemphigus disease among Iranian population." Experimental Dermatology 27.12 (2018): 1395-1398." We look at supporting information on websit in paper, especially we look for photo of Gel electrophoresis of tetra-ARMS PCR results, we want to know how the running profile of electrophoresis looks like in gel when there is/ no the rs2304365 SNP, that means what is the number of binds we are supposed to receive especially using this PCR method? Hope to get help and usefull information:)
Till now there is no known association between the ST18 gene and PV (Polycythemia Vera). Polycythemia Vera is a myeloproliferative neoplasm characterized by the overproduction of red blood cells in the bone marrow.
While genetic factors play a role in the development of PV, the specific genes involved are still being investigated. Mutations in the JAK2 (Janus kinase 2) gene, particularly the JAK2 V617F mutation, are commonly associated with PV. This mutation is found in a significant proportion of individuals with PV and leads to the activation of the JAK-STAT signaling pathway, contributing to abnormal cell growth and increased red blood cell production.
It's important to note that scientific research is continually advancing, and new discoveries about the genetic basis of diseases like PV may have emerged since my last update. To obtain the most up-to-date and accurate information on the association between the ST18 gene and PV, I recommend consulting recent scientific literature, genetic databases, or speaking with a medical professional or geneticist who specializes in the field of hematological disorders.
From the look of the inner primers which both have mismatches near the 3' end of the primers Rin-Fout is 168 bases and amplifies only the G allele while the primer set Fin-Rout amplifies a 215 base amplimer when the base is a T but it looks like the C base will not amplify with either primer set
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