I need to confirm that the antibacterial activity of the lactic acid bacterial strains is by bacteriocins(the antibacterial peptides). Are there any biochemical tests to confirm it?
Do you have it purified, in which case you can do any one of a variety of biochemical analysis.
If you are only dealing with a crude extract showing antimicrobial activity, you could show that a broad spectrum protease does or does not inactivate that activity. This would show whether it might be a protein or not. I would caution you though that some bacteriocins are protease tolerant, so if you get protease inactivation then you have confirmed it is a protein/protease but a negative result does not really tell you anything.
Organic acids are much smaller molecules than bacteriocins, so you could separate the bacteriocins from the organic acids in the medium by ultrafiltration, dialysis or gel filtration chromatography.
hello, maybe we should start by neutralizing the acidity (increase in pH) and see if there is a different effect on the activity, if there is less activity, i.e. the acid was responsible for the activity.
After you can eliminate the peptides and also compare the activity, if these peptides are responsible for the bacteriocin activity you should find less activity.
- Badges
- Science method