Either for a single gene or a list of genes
Provided that the cultured cells were supplemented with sufficient metal salts during their growth phase
17 March 2024 6,814 2 View
I want to plunge a 1cm in diameter hydrated hydrogel in ethane for fast freezing. I was wondering if anybody have experience with this and whether dealing with ethane is dangerous? Is there any...
11 December 2023 9,271 0 View
Also, my data are stationary at I(1), except the dependent variable which is stationary at I(0) and (T> N, T= 31, N= 7). But they are cointegrated. I don't know this is the best approach or not. I...
17 November 2023 8,081 2 View
Hello. I will start by explaining my research first. I just finished collecting data on the effect of object familiarity on naming performance. I did an experimental procedure. I separate...
05 May 2023 6,268 5 View
So from my understanding here are two processes of the formation/growth/repair of skeletal muscle: Myosatellite cells differentiate into myoblasts, which are proliferating. when the...
13 April 2022 271 0 View
So I have about 5-10 samples of DNA from which I amplified one specific exon that was targeted to create a mutant cell line. These samples are single-allele sequences that were obtained by...
08 April 2022 4,355 4 View
I have images of live cells taken with the EVOS bright field inverted microscope. I know they were taken with a x 10 magnification lens, but unfortunately these images have not come with a...
08 April 2022 5,929 7 View
Why does combination therapy seem to be more effective for kidney cancer? Wouldn't you have more side effects due to consuming two drugs at the same time?
17 December 2021 7,605 3 View
I am trying impose free-free boundary conditions in analyzing free vibrations of a beam. I have coded up in Matlab. I used to restrict the corresponding degrees of freedom with the related...
19 August 2018 9,727 4 View
I want to know which algorithms do you use in your integrated active chassis control system framework and do you consider the over-actuation due to four electric motors. If so, which torque...
07 May 2018 128 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
