How is possible to find out time-displacement table from cyclic loading diagram?

Hi How is possible to find out time-displacement table from cyclic loading diagram ? or is there any way available to extract this information from hysteresis plot ? I mean , i wanna extract...

13 January 2018 9,220 4 View

Why might my neurofilament IHC not be working?

I am using the Abcam antibody ab4680 on mouse brain tissue fixed via PFA perfusion. The protocol we are using is the same as in http://dx.doi.org/10.1016/j.cell.2015.11.025. It's a staining method...

23 December 2017 7,063 3 View

In your experiance with the HER have you encountered a condition/material where the reaction order vs. H+ concentration is 0.5?

I'm wondering if anyone knows any experimental scenarios where the reaction order for the HER is 0.5 (vs. pH.), either acidic or alkaline environments. It is very common to see values like 1, 1.5,...

23 December 2017 766 1 View

Normalization of histone H3 signal obtained by ChIP-qPCR?

I'm currently trying an X-ChIP-qPCR experiment to determine the H3 occupancy at some specific loci in yeast cells. I was wondering what internal control I should use. I found a lot of articles of...

28 May 2013 2,135 4 View

Im doing a linear buckling analysis, but Im not sure which mesh is my appropriate mesh. Should I have convergence in an eigenvalue problem?

and also do negative or complex eigenvalues have meaning in a buckling analysis?

01 January 1970 4,750 9 View

How to use the Modulus of Elasticity of Sand in PLAXIS?

The modulus of elasticity differs in various conditions of foundation soil. I am struggling with defining modulus of elasticity of geosynthetic reinforced sand foundation beneath a shallow strip...

01 January 1970 5,965 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View