Dear Bahare,

The optical density of a stationary phase Pseudomonas culture will vary widely and be dependent on media, temperature and oxygenation. We typically grow environmental isolates, including P. fluorescens, in King's B medium at 20°C with shaking, and get OD600 readings of 0.8 – 2.0 within 1 - 2 days. Our model pseudomonad, P. fluorescens SBW25 is very sensitive to oxygenation, so OD levels are lower after 24 h is the cultures are statically incubated, compared to a shaken incubation (and siderophore expression is also delayed). Some pseudomonads don't grow well in KB, e.g., P. syringae DC3000, while in general most o the strains we use grow well in Lauria Broth (LB).

If you want to determine the OD of the stationary phase for your strain(s) and growth conditions, then simply set up a culture and assay every 2 hr or so until stationary phase is reached.

Andrew

Hello,

For the different reasons that can influence the growth of bacteria, the best thing is that you do a study of the growth kinetics of your bacterial strain. Make a bacterial culture on liquid medium and measure the optical density at a regular time interval which will allow you to have a growth curve with the different growth phases according to the incubation time.

Hi,

Different results are obtained for different bacteria, for example, it is as follows for Pseudomonas aeruginosa:

P. aeruginosa PAO1 pre-grown to early exponential phase (OD 600 nm 0.6), mid-exponential phase (OD 600 nm 1.13), and late stationary phase (OD 600 nm 2.73,) respectively...

Reference:

Article Surfactant phospholipids act as molecular switches for prema...

Good luck.