I am looking for some insight as to how to improve my yields from performing an MNC extraction using Ficoll for cord blood units that have been cryopreserved.
The main issue is the cryoprotectant DMSO, I carried out several washes yet the cells are not separating well enough to obtain a decent yield.
I'm not sure if this helps, but according to the method on http://www.scigine.com/method.php?ID=49479, simple washes with media (not PBS) seem to remove ficoll after cryopreservation.
Maybe you can take a look at the original paper there and call or email the authors directly for help. (Paper entitled: Antiviral properties of two trimeric recombinant gp41 proteins)
Hi Romy,
Thanks for the answer, we currently do this with a large proportion of our banked units. This method of ficolling volume reduced cord blood is mainly to utilise some of the units within the tank that have already been frozen down and could be re purposed.
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