I am study a segment of a membrane protein, but it is difficult to dissolve in water. I was told to dissolve it in DMSO. It did work, but I worry if DMSO denatures protein?
Hi there,
The main issue is the concentration of DMSO you intend to use. For info:
http://www.sciencedirect.com/science/article/pii/S0301462207002050
I've used DMSO in partial factorial refolding matrices for a variety of protein classes and the effect can be dependent and independent of the other parameters in the matrix such as temperature, pH, co-solvent, redox couple, arginine, detergents, etc. It depends on the protein/domain and if you are looking for reversibility as well.
Hi,
I've tried using 20 uL of DMSO in 1000 uL reaction volume to dissolve a dye/probe for protein conjugation, and thus far there are no issues.
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