Recently we are facing this problem. We are using 20%complete medium + 10%DMSO or 90%FBS+ 10%DMSO as freezing mixture. None of these freezing mixtures are freezing even at -20. we are sure our freezer is working fine. what is going wrong. because of this we are not able to revive any cell stock
So, which is the exact temperature for you problem? -20 or -80
Is your -20 freezer auto defrost?
From my point of view we are talking about the process of supercooling.
https://en.wikipedia.org/wiki/Talk:Supercooling
https://en.wikipedia.org/wiki/Supercooling
Hi, Asavari
Did you find the problem with your medium?
We are with the same problem in our lab and I would like to know what could be happening.
Hi Luciana,
No, it is not happening with other stuff, It sounds weird but only FBS gets frozzen but freezing mix with DMSO is not.
Thak you Asavari!
So, you are still dealing with it.
If I have some news from here about it I will let you know.
Let's keep trying to understand what happens.
- Badges
- Science topic