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Questions related to Cloning
hello, I am curious about the use of RBS designer from the website: https://salislab.net/software/ how is it possible to use it for designing new RBS to overexpress protein in e.coli? thank you...
28 July 2023 6,174 2 View
i'm looking to know tehe characteritics of this applications
26 July 2023 6,200 0 View
Using immunostaining I can see the mutant protein going to the nucleus of the cell. This is also causing vacuolization. However, I am not sure if these vacuoles are causing cell death and so the...
13 July 2023 8,541 1 View
I am getting smear on gel after using linear DNA (amplicon) as a template while this is not the case with circular DNA (plasmid) as a template with same set of primers. I have used various amounts...
07 July 2023 7,215 8 View
Hello everyone. Is there anyone who is familiar with in-situ hybridization analysis from brain slices? Will be grateful for help.
06 July 2023 7,374 0 View
Hi, My CRISPR vector carries an RFP tag. I can observe fluorescence when I package the lentivirus using 293T cells. After collecting the lentivirus and determining the titer by qPCR, I...
06 July 2023 6,510 1 View
I am trying to purify a protein via Ni-NTA chromatography. The theoritical size of my protein is 58 kDa which after addition of his tag and thrombin site adds upto 2-3KDa resulting in protein band...
03 July 2023 930 0 View
Trying to clone human TOPORS gene into AAV vector plasmids. The TOPORS gene was cloned from cDNA by PCR and purified. Restriction enzyme digestion was used to isolate existing vector and make it...
26 June 2023 2,713 5 View
What is the most suitable solution for reducing pH of an solution which contains L- amino acid and peptide in a non-destructive way?
22 June 2023 8,813 1 View
I have an Arabidopsis protein, which should be apoplastic (rice homologue is localized to apoplast and this protein contains predicted signal peptide). I expressed the protein without the signal...
21 June 2023 2,352 3 View
I have read protocols for development of antibodies from rabbits post immunization with peptide. However, I shall be the first to do this in our lab. Can anyone suggest how to perform...
16 June 2023 6,619 1 View
I am working on basecalling ONT sequencing data using dorado. This is the error message: [error] toml::parse: file open error -> [email protected]_5mCG@v0/config.toml I have verified...
15 June 2023 9,242 2 View
Iam working on seaweed protein.. Iam getting protein % of 62% through kjeldahl.. But when iam calculating Protein yield, its more than 100%.. Is it possible or what can be the reason behind this?...
13 June 2023 5,890 0 View
Hello guys, I've order some siRNA from a company, and they gave the following sequence. I've tried looking up the sequence in the genome but I could not manage to find it. Does anyone knows a...
09 June 2023 8,512 1 View
Kindly suggest the best protocol
07 June 2023 9,366 0 View
I am conducting research on OT-1 murine extracted CD8T cells and their activation and proliferation. Which method is more effective for stimulating their proliferation: CD3/CD28 antibody or OVA...
06 June 2023 3,697 1 View
which titer of viral particle should be used? which concentration of lenti viral particles should be used? what volume of viral particle should be used?
05 June 2023 6,445 0 View
Good day and I hope you are all doing well. I wanted to know if Jurkat cells expressed TLR4? I have been researching these cells but it seems that they have mixed response. Some researchers claim...
03 June 2023 5,300 1 View
Hi, Has anyone have any experience using the RDML-Analysis tool after obtaining the N0 values from the RDML-LinRegPCR tool (https://www.gear-genomics.com/rdml-tools/)? I exported my qPCR RDML...
29 May 2023 2,553 0 View
Factors cause mutation
27 May 2023 4,396 4 View
please explain the process
27 May 2023 1,332 1 View
I have a cell experiment and need to use a sterile round bottom W384-well plate, but most of them are labeled as non-pyrogenic instead of sterile. Does non-pyrogenic mean sterile? If not, what is...
26 May 2023 3,479 1 View
Need 2ng/ul RNA ( from cel-mir-39) Our stock is at 1600 fMol/ul i believe its 7018.2 g/mole ( 5'- rUrCrA rCrCrG rGrGrU rGrUrA rArArU rCrArG rCrUrU rG -3' ) Please can someone explain clearly...
24 May 2023 334 3 View
I'm currently studying sex differences in a mouse model of stress-eating and am just wondering what is generally recommended in terms of housing male and female mice together for behavioural...
22 May 2023 1,803 4 View