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Questions related to Cloning
Possible solution to increase the yield of the protein?
10 February 2024 9,872 3 View
Do you have good experience with a certain equipment for synthesis and what are the costs? What would be a suitable method for purification? Are there alternatives to MS for QA? Thanks!
08 February 2024 3,602 3 View
He gets good culture and all cells look good and meet the requirement needed and explained in the protocol. But at the end, he can not get a good chromosome spread. We will really appreciate if...
07 February 2024 4,176 1 View
Does anybody know how to convert IU/ml in case of anti-TSHR antibodies (Graves disease)? I would like to know how much anti-TSHR Ab I have in a sample
03 February 2024 1,617 3 View
I was running PCR 2 lines of fax1 potential mutation (with positive and negative controls). However in the results I have not been able to detect any DNA bands in the gel electrophoresis with one...
01 February 2024 169 3 View
I'm conducting a time course study on gene expression using RT-q PCR for samples treated with 4 conditions: vehicle, RA agonist, Calcitriol agonist or a combination of both agonists over 6 hours....
27 January 2024 7,915 1 View
I want to assess multiple SNPs in multiple sample treatment groups and I am not sure which lab technique is the best for that?
25 January 2024 6,533 1 View
Hello, ResearchGate community, I am currently working on analyzing microarray data using the limma package in R. Specifically, I am using the intraspotCorrelation function for separate channel...
25 January 2024 1,956 0 View
Hello everyine, Does anybody have a proof (a review or a scietific paper) that indicates if T cells from transgenic mices OT-1 and OT-2 express CD154 on their surface? I couldnt find the answer...
25 January 2024 2,839 1 View
I would like to expand antigen-specific mouse T cell from spleen. I tried many times, but did not see any monoclonal expansion. Does anybody can help me about that? what is the culture condition?...
23 January 2024 3,705 0 View
In the determination of soluble sugar content in plant samples using the Dubois et al. (1956) method with phenol sulfuric acid, why is it advisable to utilize both a standard curve and the...
06 January 2024 7,755 0 View
To what extent can international cooperation and policy changes mitigate the effects of climate change and promote sustainable development?
03 January 2024 880 3 View
Dear esteemed colleagues, I have a conflict in the formal calculation of the concentration of sulfuric acid by the primary standard TRIS-buffer, I know from the chemical reaction that 1 mole of...
30 December 2023 1,704 0 View
How do microorganisms survive in extreme climatic conditions and what is the highest temperature bacteria can survive?
29 December 2023 4,009 2 View
Why do bacteria adapt to various conditions and how can we know whether bacteria can mutate to survive at a much higher temperature?
29 December 2023 7,814 3 View
Hi everyone, I am analysing the gene expression from different Drosphila genes by RT-qPCR. I am having problems with one of the gene, OR24a, because I see amplification in the "No RT" sample,...
27 December 2023 8,571 4 View
What kind of software are you using to analyze courtship behavior in Drosophila?
23 December 2023 7,295 0 View
We can not handle in vitro matrigel assay optimization, so that we need to have a certain useful protocol to get experiment. Can anyone share us a successfull protocol that have been already...
22 December 2023 406 0 View
I'm doing mic and mbc following the disc diffusion assay. I want to calculate the cfu value for the microbial growth in the plate. Can you please share any relevant article or the exact calculation?
21 December 2023 9,556 4 View
I want to asses percentage of dead cells taken up by macrophages. If I stain dead cells with one of Thermofishers LIVE/DEAD stains, will this be transferred to macrophages that take them up by...
21 December 2023 1,050 0 View
Hello, I'm curious about the in vitro experimental method used to assess whether or not some kinase enzymes directly phosphorylate other kinase receptors, such as EGFR. Which experimental method...
20 December 2023 9,418 2 View
Hi I am getting these weird curves on qPCR Anyone knows why ? I tried to decease the primer and tablet concentration No change.
19 December 2023 5,094 2 View
I have been reading this paper, Host Range Factor 1 from Lymantria dispar Nucleopolyhedrovirus (NPV) Is an Essential Viral Factor Required for Productive Infection of NPVs in IPLB-Ld652Y Cells...
16 December 2023 8,577 1 View
Most studies have found that hydrolase activity is positively correlated with precipitation. What perspectives can we understand that the activity of hydrolase decreases after rainfall decreases?
15 December 2023 5,193 3 View