Cloning

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Questions related to Cloning

E.coli growth standardisation with OD 600 value ?

For getting 10^9 CFU/ml of E.coli, what should be the OD600 value and hours of incubation needed in nutrient broth if we take 1-2 colonies in broth for initial incubation

15 April 2025 9,958 0 View

How do I interpret this Isothermal Titration Calorimetry (ITC) graph? Why are my heats of dilution so large?

I am trying to measure the binding between an aptamer (ssDNA) that 'supposedly' binds specifically to (+)-methamphetamine. The aptamer comes as a solid form, which I initially dilute in H20, and...

15 April 2025 5,558 1 View

What can be a reason for getting deformed mesa shape during InP/InGaAs wet etching?

I am doing wet etching of InGaAs/InP epixay using HCl:H3PO4:DI and H2SO4:H2O2:DI. I used AZ 5214 photoresist (thickness ~1.9um). I found that, while wet etching, my mesa shrinked and got deformed...

08 April 2025 6,846 5 View

Problems sequencing an insert. Any help?

I am having problems sequencing an insert: I've started from a commercial pcDNA3.1 plasmid with a target protein gene coupled to a truncated fluorescent protein gene (YFP). This plasmid has been...

03 April 2025 1,665 4 View

Hi, how can i understand which methods are the best one for MIC and MBC test based on my pothogen bacteria?

I am analyzing the antibacterial effect of my postbiotic on Streptococcus mutans as a pathogen. I found different methods with some variations; some suggest an inoculation volume of 10 or 20...

03 April 2025 9,775 3 View

Co-supervision of a thesis?

Dear Colleagues, I'm a student in the Ph.D. program, and I would like to collaborate with some researchers in the field of Industry and IA. Cordially. Younes BENALI.

02 April 2025 3,111 0 View

Seeking a collaborator in the Field of Biomedical Engineering?

Dear researchers, If your expertise exists in conducting in silico molecular docking studies, please contact me to explore potential collaborations. I am working on a project that could...

29 March 2025 413 3 View

Are environmental measurements like concentration and flow considered compositional data?

I work with environmental measurements that represent ratios of things (i.e., flow: volume/time, concentrations: mass/volume) and not absolute values (e.g., weights, mass, length, number of...

28 March 2025 3,387 2 View

Which method is suitable for characterizing MLV (Multilamellar vesicles) size, lamellarity, interlamellar spacing, and fluctuations: TEM or SAXS?

Liposome - TEM and SAXS

27 March 2025 529 0 View

I am seeking assistance in finding this research article for my PhD studies. Is there a researcher on this platform who can help me with this?

Cowley, J. M., Baker, R. T., & Harte, D. S. (1992). Definition and Determination of Host Status for Multivoltine Fruit Fly (Diptera: Tephritidae) Species. Journal of Economic Entomology,...

16 March 2025 967 5 View

¿Cuales son las principales demandas del moviento feminista hoy en día?

Las principales demandas del feminismo hoy en día incluyen la erradicación de la violencia de género, el acceso a derechos reproductivos y sexuales, la igualdad laboral y salarial, y una mayor...

10 March 2025 9,188 2 View

What measures can social workers take to prevent children and adolescents from online exploitation and abuse?

This question explores the proactive role of social workers in protecting young people from online exploitation and abuse. Calls for a review of various measures, including digital security...

09 March 2025 3,891 2 View

How to Handle Repetitive Cases in Central Composite Design (RSM) for a CFD Study in Fluent?

I am using a Central Composite Design (CCD) for my response surface methodology in a CFD study using ANSYS Fluent. However, a reviewer has pointed out that my experimental design contains several...

09 March 2025 8,967 2 View

¿Cómo pueden mejorar las políticas públicas la protección a las víctimas de violencia de género para garantizar seguridad y autonomía a largo plazo?

La pregunta lleva a reflexionar sobre cómo las políticas públicas pueden fortalecerse para brindar una protección integral a las víctimas de violencia de género, asegurando su seguridad inmediata...

09 March 2025 6,524 1 View

Western blot of tristetraproilne in spinal cord tissue after spinal cord injury in mice?

Hi all, I am trying to check the expression of tristetraproilne (TTP) in the spinal cord tissue after spinal cord injury in mice, but I am not seeing any bands for TTP at about 36 KDa...

28 February 2025 511 1 View

Why am I unable to detect IFN-γ in CD8+ and CD4+ T cells from tumor tissue using flow cytometry?

I am analyzing CD8+ and CD4+ T cells in tumor tissue using flow cytometry but cannot detect IFN-γ. I did not use any stimulation. Reagents I used were cytofix, perw/wash buffer, cell staining...

26 February 2025 3,095 4 View

What is the minimum voltage to place in the power source to generate electrophoresis in oral mucosa cells?

18 February 2025 6,781 1 View

Cual es el voltaje minino a colocar en la fuente de poder para generar electroforesis en células de la mucosa bucal?

18 February 2025 9,736 1 View

Why did HEC-1-B cells stop proliferating?

I passaged HEC-1-B cell lines 4 times successfully for the experiments and then unfortunately very little cells left for the T-25 flask. For 10 days, the cells have neither died nor proliferated....

14 February 2025 2,808 2 View

Is it possible to calculate the amount of Lewis acidity & Bronsted acidity in mol/g using py-adsorbed FTIR spectra?

If it is possible, then how it is calculate?

13 February 2025 398 1 View

How to find the translational potential of circRNA?

It's been 5 days and I am trying to find the translational potential of certain circRNAs, but since CircRNADb is not working anymore and without any proper paper mentioning that the circRNA has...

09 February 2025 8,654 1 View

IS the entropy generation in double diffusive convection increase or deserease with Ha ?

explain your idea

08 February 2025 9,774 2 View

What is social media?

what is social media and its details like how it changed the life of individuals

07 February 2025 8,379 2 View

Can I predict the interaction between nucleic acid structure and protein structure based on their 3D sturcture (*PBD files)?

Can I predict the interaction between nucleic acid structure (eg. G-quadruplex) and protein structure (eg. G-quadruplex helicase) based on their 3D sturcture (*PBD files)? Wether the "AutoDock"...

05 February 2025 9,005 3 View

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