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Questions related to Cloning
how to green synthesis of nanoparticles by using plant extract?
03 September 2024 8,949 2 View
I fixed bacterial cells in 2.5% glutaraldehyde and dehydrated them in a series of ethanol solutions, ending with 100% ethanol. I intended to proceed with critical point drying (CPD), but the...
28 August 2024 1,053 5 View
I have three input (A, B, C) and one output (E) with standard deviation in measurement. How to train levenberg marquadrt back propagation algorithm
22 August 2024 7,342 0 View
Hello, I am studying autophagic activity by using LC3B marker. I already know that my patient cell lines have higher levels of LC3BI and II forms compared to the control. However, these data by...
17 August 2024 7,278 3 View
Factors: 3 numeric factors 2 categoric factors Goal: I want to have a design where the values of my numeric factors always sum to a constant value (for example A+B+C = 100, where A, B, and C are...
12 August 2024 2,215 1 View
Hello, I want to show cell % of cells with a specific mRNA expression. so I did the staining with my targeted protein once with mRNA C and another with mRNA D. I used Qupath to get the cell...
12 August 2024 2,546 3 View
I did a cross of flx/flx mouse with flx/flx/vmd-cre mouse. In starting, I standardized everything and got both type of genotypic mice (pure flx/flx) and (flx/flx/vmd-cre mouse), till 3...
08 August 2024 6,086 0 View
Assistance Needed: Dual Culture Assay Issues for Chilli Anthracnose disease Project Dear Researchers, I hope this message finds you well. I am currently working on a project focused on chilli...
06 August 2024 9,806 3 View
I need to publish my work on two projects based 1. molecular identification 2. Retrospective analysis
22 July 2024 5,404 0 View
Hello, I am going to generate knock-in zebrafish model for my gene using Bi-FoRe plasmid. the gRNA target site for my gene has 43 percent efficiency. so i did microinjection using 600ng of Cas9...
09 July 2024 9,019 0 View
Hey, I have been trying to transduce cardiac fibroblasts with lentiviruses. I transduced them using different concentrations of non-concentrated as well concentrated viruses. I went for as high as...
08 July 2024 7,126 5 View
Is the way of using Machine Learning and Artificial Intelligence to detect foliar and telial plant diseases the same when using plant images? Is it the same procedure and/or the same algorithms?...
05 July 2024 9,487 2 View
I want to knock out a gene using CRISPR/Cas9 in detached chili leaves. I used Agrobacterium to insert the CRISPR/Cas9 system using syringe infiltration and incubate in 28 C for 2 days. The PCR...
02 July 2024 624 0 View
I am doing extraction miRNA from sputum samples, by using extraction kit, but the problem is low concentration of miRNA. I have tried adding PBS 2-5 times into the sample before the extraction,...
02 July 2024 3,810 2 View
I want to analyze some images (nearly 1000) in a loop. I want to analyze HSV and RGB. I have masked those images in ImageJ, which is binary masked. I tried to explore them in R, but all the...
05 June 2024 5,202 1 View
Dear colleagues, I'm planning to start neuron mouse co-culture with astrocyte and microglia but I heard that it's difficult to keep it up. Any advices?
28 May 2024 5,054 3 View
I try to understand and identify my dsDNA structure. I need to know if my DNA has a special structure and it is double-stranded or not. Which method should I use ? Are FTIR and CD sufficient ? I...
27 May 2024 5,791 1 View
Hi I have been working on droplets, lipid vesicles for my experiment and I have a serious issue that my droplets get stuck on the coverslip. I use the following protocol below to make my...
22 May 2024 9,374 3 View
--Hi, such as RTqPCR to analyse gene expression, is it necessary to have biological replicates in a ddPCR experiment ? thank yu --
22 May 2024 8,264 6 View
How information technology can contribute to overall environmental sustainability and role of technology in promoting environmental sustainability in India?
21 May 2024 9,209 6 View
I have harvested human dermal fibroblast cells and subcultured into T25 flasks using fibroblast expansion medium that contant 10% LSGS and 1% anti-anti. When observe the cells a day after...
17 May 2024 8,307 0 View
I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? 2. I designed as RBS located after his...
11 May 2024 7,633 2 View
Hello fellow researchers, I am currently encountering significant challenges in a classical cloning procedure and am in need of your expertise. Here is a brief overview of my...
04 May 2024 7,736 5 View
dear all can you suggest where can i refer to the ppar agonist? where do we purchase the molecules?
03 May 2024 483 1 View