2/1/24

Dear Jasmine,

Each of your gels has a single "lane" with bands. I assume that these are standards. What are you using to stain your gels? Are you visualizing the DNA w/ a dye or by using a UV lamp?

I don't work w/ DNA, but I have done gel electrophoresis of proteins. When I couldn't see bands in the gel, it was usually because the amount of protein in my sample was so low that it couldn't be visualized by the protein stain. This was solved by adding a larger amount of sample. This may be your problem. Is there any way that you can add more sample to the gel? Another option is to mix a little of your protein standard solution with your DNA sample and apply the mixture to the gel. It is possible (but unlikely) that something in your DNA sample is preventing visualization. This is probably not the solution, but it's easy to try. I suspect that sample size is the source of your problem.

I hope this information helps you. Good luck w/ your research.

Bill Colonna

Dept. Food Science & Human Nutrition, Iowa State University, Ames, Iowa, USA [email protected]

By looking at your gel electrophoresis result, where there is no band observed, and you already use positive control. Most likely the problem is in your PCR reaction components, try again using a fresh reagent.

you have a large primer dimer blurred band which removes primer from the pcr.Try using less primer and use a hot start polymerase. Also check the od260/280 of you dna to check that its quality is good and that you are not adding too much dna to your pcr and poisoning the pcr reaction