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for example the principle for quantification of alkaloids is based on formation of alkaloids precipitate by ammonium hydroxide after separation from its salts.
22 May 2023 9,478 1 View
Hello. I am trying to transduce the cHL and NHL cell lines (L428, Ca46, Ramos, U2932) with lentivirus with expression vector CD550a1 with miRNA insert. The transduction efficiency is very low but...
18 May 2023 910 0 View
Hi, I need to amplify a tRNA fragment in a normal PCR, The primers I designed suppose to give me a product of 40 base pairs. I want to transform it into pGEM and send it to Sanger sequencing . My...
04 May 2023 5,356 3 View
Hi all, I have many stable cell clones that originated from single cells. However, I found (by IF) that not every cell expresses the protein, and this percentage varies between clones. I was...
04 May 2023 5,761 3 View
Dear Researcher, I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am, I found many protocols using probenecid , My question: is it ok if i...
18 April 2023 6,474 0 View
We are in the process of determining the title and making one-step growth curves for lytic phages, but we are at a point where dilutions up to 10ˆ-54 result in complete lysis.... no counting of...
18 April 2023 770 4 View
Hi, I have been reading about vectors and strategies used in engineering insect cell lines. PiggyBac being a very promising system, is not supplied with a autonomously replicating transposase...
18 April 2023 5,900 6 View
i have detected alkaloid, phenol and tannin from my bacteria sample than i did hpltc. as standards were not available i am unable to detect the exact compound from tthe peak. can any one suggest...
08 April 2023 668 0 View
Hi, I am working with Arabidopsis and want to do ChIP-seq. I isolated chromatin from 14-day old seedlings and did sonification. After reverse crosslinking with 4 microliter of NaCL 5 M (ON), 2...
05 April 2023 6,819 0 View
Hi everyone. I have experience using the CRISPR-Cas9 system to establish knockout cell lines. Now, I am planning to introduce a single point mutation into my gene of interest using the CRISPR...
05 April 2023 8,743 4 View
Hi Everyone! Richard Arthur Buckingham was the originator of the Buckingham potential formula. Although this potential is implemented in several MD codes, I haven't found online any picture about...
04 April 2023 9,325 0 View
Hello everyone, I am currently seeking a co-author for a manuscript submitted to the Journal of Biomolecular Structure and Dynamics. The manuscript presents a study on biomolecular conformations,...
27 March 2023 535 1 View
I am measuring the expression of a fluorescent protein over a period of 4 hours (15 min intervals), testing 4 different conditions with 2 control groups (one positive for expression of the...
24 March 2023 2,020 4 View
Has anyone ever successfully used mouse serum as the sample in a Western blot? In this case, could you give me some tips? I have home-brew mice polyclonal sera and I tried to check it in WB. Using...
24 March 2023 1,691 0 View
Hello, I am having an issue with my PCR gels, when using a gel with two rows, the samples on the bottom row appear very faint or not at all. The bands from the top row appear nicely, as does the...
24 March 2023 7,128 3 View
I want to insert the km resistance gene into the ampc gene in E.coli. I want to do this with Datsenko and Wanner method. I amplify the km gene from the pkd4 plasmid with a 40 base tail at the...
21 March 2023 5,897 1 View
Hello, I am using Word 2019 Insert Citation option for citing inside the text. I want to change the color of appearance of the citation in the text. How to do that? And I am using APA style of...
18 March 2023 4,201 0 View
I am ligating gene of interest between EcoRI and HindIII within pET28(b+) plasmid. What could be the best incubation temperature and time for the ligation to proceed?
15 March 2023 2,326 3 View
I need to concentrate some fatty acid methyl esters in a cell culture medium to separate and isolate them using Prep-HPLC. What type of solid phase extraction would be suitable to concentrate...
03 March 2023 9,567 0 View
Can we use mammalian antibodies for crustaceans' proteins in western blot analysis?
02 March 2023 2,463 3 View
I am looking for protocols on tRNA measure using qPCR, does anyone have suggestions on how to quantify tRNA using qPCR in mammalian cell? AND how to design the qPCR primers specific for tRNAs? I...
15 February 2023 3,374 0 View
Hello all, I need urgent help. I have to prepare incomplete media whic includes '2.5 uM BSA' in it. How should i prepare stock ? As I am going to use BSA in incomplete media can i thaw the BSA...
07 February 2023 582 1 View
Does anybody here know how to use ImageJ to determine the OD sections of Luxol Fast Blue stained? I need aid. I wanna compare the spare myelin in each group quantitatively using dyed spinal cord...
20 January 2023 8,860 1 View
Hi! We would like to do a CRISPR screening using cell lines modeling mouse neurons and astrocytes. I guess ideally the cell line needs to be easily transfected/transduced and easy to handle. I'm...
14 January 2023 1,224 0 View