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Questions related to Cloning
Hi, I have a project that requires homology-directed repair using CRISPR to add a sequence. I used the Benchling tool and figured out all the components such as HR arms and gRNAs. It even shows...
25 September 2023 2,004 1 View
In literature, articles claimed that There is no evidence to suggest that repeated vaccinations weaken the overall immune system. In fact, they can enhance your immune system's ability to respond...
24 September 2023 7,445 4 View
I am working with iPSC cell culture and finding contamination after two or three days. Media color changes yellowish green and turbid. I was not able to see any cells after the contamination even...
22 September 2023 1,911 7 View
Hi I'm purifying some mutants of the protein I study. The wild type protein exists as a monomer and is 28kDa. I have two mutants (same protein, same number of amino acids but with 8 amino acid...
22 September 2023 10,015 5 View
I recently performed protein extraction with RIPA buffer of some cells I collected. The intended purpose was to use it to load in an SDS PAGE gel for subsequent Western Blot. I made the mistake of...
21 September 2023 7,618 4 View
Random error is the accumulation of an infinite number of variables, and because the sum of an infinite number of variables follows a normal distribution according to the central limit theory, the...
21 September 2023 7,786 0 View
Most questionnaires follow a normal distribution if they are collected properly and an appropriate normal distribution test is used. Determine the goal of whether the questionnaire has a normal...
21 September 2023 9,568 0 View
I'm currently working with human primary T cells, isolated from whole blood that I get from my university's blood bank, and I'm trying to determine if my experimental treatment can enhance...
20 September 2023 9,070 2 View
We have developed a fetal bovine serum product that is produced concurrently with the process of fibrin production. We are intrigued by the potential commercial applications of this fibrin. Can...
20 September 2023 2,798 0 View
I'm analyzing Botrytis cinerea leaf infections on common bean plants, and for that I'm looking for a software to: 1. Measure total leaf area 2. Measure infected leaf area I've so far used ImageJ,...
20 September 2023 4,132 1 View
Hello, I need to prepare 50mM sodium acetate buffer, pH 5. I figured that I need to prepare 50mM sodium acetate (adding around 820,3 mg to 200ml water) and then add 50mM acetic acid (prepared by...
19 September 2023 7,346 1 View
Is it possible that in a co-infected host cell, DNA of a recombinant AAV integrates into the DNA of a wild-type adenovirus with non-homologous end-joining? Thus, may the wild-type adenovirus...
18 September 2023 4,663 3 View
He has sufficient knowledge of ready-made statistical applications and data analysis methods
18 September 2023 2,780 2 View
Statisticians have a major role in the work of companies and institutions of various types and specializations
16 September 2023 4,684 0 View
I am currently trying to identify gram-negative bacteria from my 16S metagenomic sequencing data, whose OTU/ASV is picked by Usearch. Somehow I found the OTU table can't be operated by BugBase...
15 September 2023 623 1 View
I inserted my D-product artificial-miRNA into pDONR207 DH5alpha, colony confirmed with PrimerA , PrimerB also with attB1, and attB2 primers. similarly, the plasmid was confirmed with the same...
15 September 2023 6,136 3 View
Which is the bioinformatic tool useful to evaluate if a specific cytosine is present in a response element or known enhancer/repressor region? E.g., differentially methylated cytosine in...
13 September 2023 6,369 2 View
Hello every one, I'm working on the in vitro expression regulation of a viral gene. I'm looking at the splicing efficiency of several versions of the gene (comming from different genotypes). To...
11 September 2023 9,678 1 View
How to extract protein efficiently
08 September 2023 5,997 1 View
I use imageJ to quatify protein but there are too many Subjective factors
08 September 2023 7,137 3 View
Hello, I am trying to ligate a fragment of 4.7 kb into pENTR4 dual selection vector with one restriction enzyme only. I performed the digestion reaction with NotI, then I purified the...
07 September 2023 6,790 0 View
Hello all, Recently I have noticed an uptick of instances where my miniprepped plasmids, when sequenced (using Plasmidsaurus), often give back either 2 peaks or only one peak, where this peak is...
07 September 2023 4,852 2 View
I am working on simple Y maze these days. I observed that most of my disease mice didn't show any spontaneous alteration in the experiment? However, some of the mice show some alteration. Could...
06 September 2023 1,529 2 View
Deterioration of the inhibitor Beta-lactamase produces strains of E.coli, what are the characteristics of beta-lectamase that helps grow E.coli and what antimicrobial agents can be used to lessen...
05 September 2023 511 2 View