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Questions related to Cloning
(1) SHARPILO, V. P. 1961. New nematode Spauligodon saxicolae nov. sp. Parasite of the scaly lizard Lacerta saxicola Evers. Trudy Ukrainskoe Respublikanskoe Nauchnoe Obshchestvo Parazitolohov 1:...
03 May 2024 4,289 0 View
kindly if you have any idea about this question leave an answer, any simple applied idia can help.
30 April 2024 1,186 6 View
"Becoming Wolf" is a study of cinematic werewolves that bears on human identity. It is about 6600 words long and contains 7 black/white illustrations, 300 dpi.
23 April 2024 3,605 1 View
Hello everyone, I work with skin cells and am performing RT-qPCR of primary fibroblast cells and primary keratinocyte cells to further use as a comparison to iPSC-derived skin cells and 3D skin...
23 April 2024 6,324 2 View
Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...
22 April 2024 3,352 0 View
Hi, I have transfected my HEK293 cells with pcDNA3.1_plasmid of interest with EGFP by using lipotransfection method. Post transfection 48h the egfp expression looks good with more than 50%...
21 April 2024 2,624 2 View
I have a important question to analyze the result of methylation percentage, but when we analyze the methylation result we focus in one DNA strand out of the two strands it has, however, we want...
20 April 2024 6,402 3 View
I would like to receive details on diatoms and foraminifera, especially information such as biostratigraphy, paleoecology and paleoenvironment.
12 April 2024 4,945 1 View
I would like to know if it is truly significant and if it can also be another cause of the increase in LD levels and hemolysis index in SE and HP blood tubes.
11 April 2024 7,708 1 View
Good morning, I urgently need to perform ddPCR analysis on some samples using a QX200 Droplet Reader that's been sitting in my lab for a while. A technician came in last month to set it up and I...
10 April 2024 4,483 2 View
Hello seniors, The suicide plasmid pRE112 was subjected for gene knockout construction. However, after several attempts the negative control (linear...
10 April 2024 8,934 0 View
phenol chloroform extraction method is used for purification of nucleic acids DNA/RNA
04 April 2024 8,432 0 View
phenol chloroform extraction method is used to purify nucleic acids to obtain a good concentration.
04 April 2024 7,398 0 View
What are cell bio techniques to identify RNA-DNA hybrid(r loop)?
03 April 2024 1,881 1 View
There are many spots on the tomato fruits, the size of a pinhole in the middle, and sometimes there is a white ring outside. Please help us and identify this pathogen. Thanks a lot!
02 April 2024 5,238 2 View
The training received by child protective services workers has gaps in transition to casework with children and families.
02 April 2024 4,189 1 View
Please, does anyone know how to bind Concanavalin A to Sepharose beads to form a gel for the mannan removal (Affinity chromatography)?
02 April 2024 2,960 0 View
Fractionation 1. Scrape cells and collect by centrifugation (500 × g, 5 min, 4 °C) 2. Resuspend cell pellets in lysis buffer and incubate on ice for 10 minutes 3. Centrifuge at 500 x g for 5 min...
26 March 2024 6,512 3 View
Bands are appearing very, very faint! I presume low DNA in gel, but my DNA concentration is over 1ug/ul. This is after my overnight digestion. I used a MaxiPrep protocol to extract DNA from...
26 March 2024 3,716 3 View
Currently i am working on a system of i4/mmm ( material composition is AB2C2) class Which corresponds to ibrav =7. I have sourced my CIF file from crystallographic open database, have crossed...
25 March 2024 536 3 View
Hi all! I can't dive into details, but I'm having issues with neutralising sodium citrate pH 5 in a 2-step cDNA synthesis and final RT-PCR. I'm manipulating my reverse transcriptase (100uL...
25 March 2024 7,331 0 View
What materials are used? What makes it so expensive?
22 March 2024 4,064 2 View
I am expressing dsRNA using E.coli as vector. I would like to check that my bacteria culture is expressing dsRNA. I am planning to extract RNA from my bacteria culture and then load the total RNA...
19 March 2024 1,517 3 View
Hi, This is E. Park. I'm trying to purify a protein which was produced in E. coli as inclusion bodies. I already solubilized the inclusion bodies using a 6M guanidine-HCl buffer. Here is my...
19 March 2024 3,825 1 View