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Questions related to Cloning
I want to analyze some images (nearly 1000) in a loop. I want to analyze HSV and RGB. I have masked those images in ImageJ, which is binary masked. I tried to explore them in R, but all the...
05 June 2024 5,041 1 View
Dear colleagues, I'm planning to start neuron mouse co-culture with astrocyte and microglia but I heard that it's difficult to keep it up. Any advices?
28 May 2024 4,869 3 View
I try to understand and identify my dsDNA structure. I need to know if my DNA has a special structure and it is double-stranded or not. Which method should I use ? Are FTIR and CD sufficient ? I...
27 May 2024 5,656 1 View
Hi I have been working on droplets, lipid vesicles for my experiment and I have a serious issue that my droplets get stuck on the coverslip. I use the following protocol below to make my...
22 May 2024 9,229 3 View
--Hi, such as RTqPCR to analyse gene expression, is it necessary to have biological replicates in a ddPCR experiment ? thank yu --
22 May 2024 8,136 6 View
How information technology can contribute to overall environmental sustainability and role of technology in promoting environmental sustainability in India?
21 May 2024 9,104 6 View
I have harvested human dermal fibroblast cells and subcultured into T25 flasks using fibroblast expansion medium that contant 10% LSGS and 1% anti-anti. When observe the cells a day after...
17 May 2024 8,205 0 View
I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? 2. I designed as RBS located after his...
11 May 2024 7,518 2 View
Hello fellow researchers, I am currently encountering significant challenges in a classical cloning procedure and am in need of your expertise. Here is a brief overview of my...
04 May 2024 7,616 5 View
dear all can you suggest where can i refer to the ppar agonist? where do we purchase the molecules?
03 May 2024 373 1 View
(1) SHARPILO, V. P. 1961. New nematode Spauligodon saxicolae nov. sp. Parasite of the scaly lizard Lacerta saxicola Evers. Trudy Ukrainskoe Respublikanskoe Nauchnoe Obshchestvo Parazitolohov 1:...
03 May 2024 4,070 0 View
kindly if you have any idea about this question leave an answer, any simple applied idia can help.
30 April 2024 925 6 View
"Becoming Wolf" is a study of cinematic werewolves that bears on human identity. It is about 6600 words long and contains 7 black/white illustrations, 300 dpi.
23 April 2024 3,451 1 View
Hello everyone, I work with skin cells and am performing RT-qPCR of primary fibroblast cells and primary keratinocyte cells to further use as a comparison to iPSC-derived skin cells and 3D skin...
23 April 2024 6,143 2 View
Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...
22 April 2024 3,163 0 View
Hi, I have transfected my HEK293 cells with pcDNA3.1_plasmid of interest with EGFP by using lipotransfection method. Post transfection 48h the egfp expression looks good with more than 50%...
21 April 2024 2,428 2 View
I have a important question to analyze the result of methylation percentage, but when we analyze the methylation result we focus in one DNA strand out of the two strands it has, however, we want...
20 April 2024 6,213 3 View
I would like to receive details on diatoms and foraminifera, especially information such as biostratigraphy, paleoecology and paleoenvironment.
12 April 2024 4,783 1 View
Good morning, I urgently need to perform ddPCR analysis on some samples using a QX200 Droplet Reader that's been sitting in my lab for a while. A technician came in last month to set it up and I...
10 April 2024 4,027 2 View
Hello seniors, The suicide plasmid pRE112 was subjected for gene knockout construction. However, after several attempts the negative control (linear...
10 April 2024 8,730 0 View
phenol chloroform extraction method is used for purification of nucleic acids DNA/RNA
04 April 2024 8,228 0 View
phenol chloroform extraction method is used to purify nucleic acids to obtain a good concentration.
04 April 2024 7,207 0 View
What are cell bio techniques to identify RNA-DNA hybrid(r loop)?
03 April 2024 1,755 1 View
There are many spots on the tomato fruits, the size of a pinhole in the middle, and sometimes there is a white ring outside. Please help us and identify this pathogen. Thanks a lot!
02 April 2024 4,861 2 View