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Questions related to Cloning
If i want to run an elisa with antigen coated plates and use serum as my analyte and anti-human ab as secondary antibody is it possible to compare the signal to a standard curve of generic/...
23 September 2025 9,822 3 View
Hi, I recently started culturing hESCs (H1 cell line for neural differentiation). During maintenance of the H1 cell line, the cells abruptly turned brownish in color and formed round EB-like...
16 September 2025 3,923 2 View
impacts of stem cells on pathogenic parasites.
15 September 2025 9,930 3 View
0.01 mg/ml fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin.
05 September 2025 1,877 2 View
What role can integrated nutrient and pest management play in enhancing the productivity and resilience of field and horticultural crops under climate change scenarios?
04 September 2025 8,481 3 View
I need some up-to-date articles on the topic of "Taking care of the couple's relationship in order to consolidate marital peace and reconciliation." What I mean is that after a couple who filed...
25 August 2025 4,462 3 View
I am working with very limited tissue/cell samples and need to analyze both mRNA (RT-qPCR) and protein (Western blot) from the same sample. Is there an efficient protocol that allows sequential...
19 August 2025 732 4 View
In publication "A new low-cost method for long-term preservation of filamentous fungi" by Osama A. Al-Bedak, Rania M. Sayed, Sedky H.A. Hassan, a method for preserving fungi on PDA infused cotton...
11 August 2025 5,177 4 View
Any unique idea that we can add in image recognition using deep learning or machine learning by preprocessing the image and then training and testing on it. It is general but something new to add.
07 August 2025 4,668 3 View
I am testing fungicides on plant pathogenic fungi, by using microplate spectrophotometer assay with OD measurements and use AUC (area under the growth curve) to compare test wells with positive...
17 July 2025 2,672 1 View
Triazole is suitable to isolate miRNA, especially if miRNA is less than 200 nt.
14 July 2025 7,527 4 View
I am culturing Vero cells and have noticed large vacuoles in them. Does this indicate aging? What other signs of aging are there in animal cell cultures? [Questions are selected from...
11 July 2025 4,425 3 View
Has anyone successfully maintained murine plasmacytoid dendritic cells directly isolated from mouse spleen or tumors in culture? It seems like the literature only looks at pDC derived from common...
08 July 2025 7,041 2 View
What are the physiological limits of delaying harvest in tree fruits without compromising nutritional value?
06 July 2025 5,520 1 View
Hello colleagues, I am working in SuperPro Designer to model a continuous bioprocess that starts with a batch phase and then switches to continuous kinetic fermentation. My objective is to...
04 July 2025 546 1 View
I am looking for information on the protein labeling process for Sulfo-Cyanine7 (CY7, HY-D0825). Does anyone have insights or resources that could guide me through this procedure? Thanks in advance!
02 July 2025 1,009 2 View
I want to extract miRNAs from plant extracellular vesicles without using a kit. The protocol I found is for human extracellular vesicles and uses glycogen. Can anyone guide me?
26 June 2025 2,590 3 View
Hi Recently we are facing problem of developing white precipitate in MDA-MB 231 cells cultured in DMEM obtained from NCSS, Pune within two day of incubation in 5% CO2 incubator or L15 media...
26 June 2025 2,839 3 View
What are the articles from the Journal of Vibration, Measurement and Diagnosis?
19 June 2025 967 1 View
I want to create hypoxia with cocl2.6h20, should I subtract the molecular weight of sun or should I use the molecular weight of cocl26h2o?
19 June 2025 2,193 0 View
When I used the β-Galactosidase staining kit (HY-K1089, MCE) for cell senescence detection, the expected positive signal (such as blue particles) did not appear at all. I used induced senescence...
17 June 2025 8,682 1 View
As part of my bachelor thesis, I am doing CFD simulations of miniature turbines in the Ansys fluent software. In the output report, I request the moment applied to the turbine wall as well as the...
13 June 2025 5,648 4 View
I am trying to leach out Fe from the core of Fe/Graphite particles. graphite was synthesized around Fe so it’s not natural graphit. I’ve tried different oxidation and acid treatments and graphite...
12 June 2025 866 2 View
I am transfecting MDCK cells and wondering if there are any things to look out for using this transfection reagent, or if anyone has any positive or negative reviews using this reagent.
11 June 2025 4,414 1 View