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Questions related to Cloning
In the determination of soluble sugar content in plant samples using the Dubois et al. (1956) method with phenol sulfuric acid, why is it advisable to utilize both a standard curve and the...
06 January 2024 7,704 0 View
To what extent can international cooperation and policy changes mitigate the effects of climate change and promote sustainable development?
03 January 2024 843 3 View
Dear esteemed colleagues, I have a conflict in the formal calculation of the concentration of sulfuric acid by the primary standard TRIS-buffer, I know from the chemical reaction that 1 mole of...
30 December 2023 1,661 0 View
How do microorganisms survive in extreme climatic conditions and what is the highest temperature bacteria can survive?
29 December 2023 3,975 2 View
Hi everyone, I am analysing the gene expression from different Drosphila genes by RT-qPCR. I am having problems with one of the gene, OR24a, because I see amplification in the "No RT" sample,...
27 December 2023 8,536 4 View
What kind of software are you using to analyze courtship behavior in Drosophila?
23 December 2023 7,269 0 View
We can not handle in vitro matrigel assay optimization, so that we need to have a certain useful protocol to get experiment. Can anyone share us a successfull protocol that have been already...
22 December 2023 371 0 View
I'm doing mic and mbc following the disc diffusion assay. I want to calculate the cfu value for the microbial growth in the plate. Can you please share any relevant article or the exact calculation?
21 December 2023 9,524 4 View
I want to asses percentage of dead cells taken up by macrophages. If I stain dead cells with one of Thermofishers LIVE/DEAD stains, will this be transferred to macrophages that take them up by...
21 December 2023 1,023 0 View
Hello, I'm curious about the in vitro experimental method used to assess whether or not some kinase enzymes directly phosphorylate other kinase receptors, such as EGFR. Which experimental method...
20 December 2023 9,385 2 View
Hi I am getting these weird curves on qPCR Anyone knows why ? I tried to decease the primer and tablet concentration No change.
19 December 2023 5,061 2 View
I have been reading this paper, Host Range Factor 1 from Lymantria dispar Nucleopolyhedrovirus (NPV) Is an Essential Viral Factor Required for Productive Infection of NPVs in IPLB-Ld652Y Cells...
16 December 2023 8,536 1 View
Most studies have found that hydrolase activity is positively correlated with precipitation. What perspectives can we understand that the activity of hydrolase decreases after rainfall decreases?
15 December 2023 5,156 3 View
I am trying to ligate a 3Kb band isolated insert to a 12.2 kb vector and I am having issues with transformations. I hardly see colonies on the plates and even if they are formed it is very less....
15 December 2023 9,272 0 View
Hello, I have conducted a bacteria challenge test on tilapia, but I found that I got LD50 negative during calculation. what it means?
14 December 2023 6,850 5 View
I am currently conducting research on RSV, and I am eager to know if the number of freeze-thaw cycles affects the titer after amplifying the RSV virus using HEp2 cells. Multiple freeze-thaw cycles...
13 December 2023 790 1 View
for educational purpose.
12 December 2023 4,659 5 View
Suppose I want to prepare 0.1M sodium citrate buffer of pH 3.0, I observed that most people use citric acid monohydrate (as weak acid) and sodium citrate dihydrate (as sodium salt of weak acid)...
12 December 2023 8,219 3 View
Hello dear scientists How can we distinguish maternal contamination from triploidy in QF-PCR analysis?
10 December 2023 9,466 2 View
How to go about testing (in-vitro & in-vivo) the anti-cancer or anti-proliferative activity of a natural plant extract compound against bone/osseous cancer. And which bone cancer cell lines...
08 December 2023 2,168 2 View
I am trying to clone ~3.8kb insert into a ~4.7kb plasmid vector. Things are fine upto transformation. However, during screening when I am isolating the plasmid, I am getting only one...
08 December 2023 6,346 3 View
The liver sections look fragmented, how could the histological technique be improved to better observe this tissue under the microscope?
07 December 2023 7,336 3 View
I am seeking clarification on the differences between the two commercial baculovirus systems for insect expression: Bac-to-Bac system (Invitrogen)and the MultiBac system (from by Imre Berger and...
07 December 2023 2,562 1 View
what is the reason behind this?is it due to the cDNA containation? usually how much cycles we are considering for doing PCR ?
07 December 2023 1,132 0 View