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Questions related to Cloning
I need some help with deciding the next steps for my experiment and would like to see what everyone has done. We have developed an mRNA vaccine we would like to test in vitro. I have chosen...
21 November 2024 6,562 1 View
Flow cytometry is an effective tool for detecting apoptosis, helping researchers distinguish between live cells, apoptotic cells, and necrotic cells. This guide describes how to use flow cytometry...
15 November 2024 6,044 0 View
I wanna optimise a process condition where I will use 3 extraction parameters how I write Rstudio code for RSM. #R#Rstudio
09 November 2024 9,753 4 View
what are contributing factor in vicarious trauma in child welfare social worker?
08 November 2024 6,088 3 View
please insight into this topic
08 November 2024 7,257 2 View
Hi everyone, I am starting to work with extracellular vesicles from mesenchymal stem cells cultured with Messencult ACF-Plus medium. The technique used for their isolation is...
17 October 2024 5,956 4 View
As a new user of the CellTrace Violet (CTV) Proliferation Kit, I'm questioning if its really necessary to purchase a new kit for each isolated experiment, since the protocol says to "Use stock...
15 October 2024 6,426 2 View
Try Explain
14 October 2024 3,311 7 View
Hi, Is the applied biosystems miRNA cDNA synthesis kit https://www.fishersci.co.uk/shop/products/taqman-advanced-mirna-cdna-synthesis-kit-1/15451034 specific to miRNAs only. i.e if I have a...
09 October 2024 7,930 1 View
Recently found a processed pseudogene in which exon 1 was not present. Happy to hear possible explanations.
09 October 2024 8,578 1 View
I am currently working on a project focused on gastrointestinal (GI) parasites in donkeys, Sri Lanka. We have collected stool samples and identified various eggs of GI parasites. However, we would...
08 October 2024 896 2 View
I have increased the FBS content to 20%, after 3-4 days of observation the MDA-MB-231 cells are not attaching to my flasks. I'm using DMEM high glucose media for this. Can I have some suggestions...
07 October 2024 3,837 2 View
Hello everyone. In my project, I am dealing with a siderophore binding protein and I need to calculate the dissociation constant of the protein with various siderophores and its precursors with...
05 October 2024 4,622 1 View
التعرف إلى العناوين المطروقة حديثاً في مجال مناهج التدريس للأطفال
30 September 2024 5,881 13 View
التعرف إلى العناوين المطروقة حديثاً في مجال طرائق التدريس للأطفال
30 September 2024 9,573 3 View
Milliseconds matter: Biomechanical inverse dynamics analysis is highly sensitive to imperfect data synchronization In biomechanical inverse dynamics, small timing errors in data synchronization...
27 September 2024 2,569 1 View
Hi there. I have used lentiviral-based shRNA gene knockdown successfully in the past, achieving >80% downregulation in target-gene expression. However, more recently, my knockdown efficiency...
26 September 2024 5,669 3 View
We have problems in binding the protein to the Streptactin resin, possibly nickel excess can compete or hinder the binding? Anybody had similar problems? Thanks Francesca
25 September 2024 5,478 0 View
How to do Synthesis of Nano biochar using Plant Resources?
21 September 2024 4,604 2 View
Just i need the protocol for development of mycorrhiza strain
16 September 2024 6,669 1 View
Hello, I have used EdU and IHC simultaneously in the past, but only for fluorescent IHC. I am wondering if it is still possible to do with chromogenic IHC and if so, should EdU be done before or...
11 September 2024 7,484 0 View
Can we calculate radical contribution using initial reaction rates instead of rate constants in UV/oxidant systems, particularly if the reaction shows unusual behavior at high concentrations of...
11 September 2024 8,040 3 View
Hello I ran a parallel Mediation analysis using PROCESS Model 4, but the specific indirect effect of M2 is throwing me off. X = Child sexual abuse; Higher scores mean more severe CSA M1 =...
09 September 2024 2,298 2 View
In the identified case of familial desminopathy (T341P DES mutation in heterozygous state), the son has bradycardia, but the father did not have bradycardia. How can this fact be explained?
08 September 2024 8,695 15 View