Hi,

This question has been asked by many scientists in different ways, my question might be a modification of the same.

I have been analysing RNA-Seq data on Galaxy with Limma-voom and used two logFC cut-offs, +1/-1 (FC=2) and +0.5/-0.5 (FC=1.5). I have identified interesting genes with the 0.5 threshold, and want to use this threshold for my downstream analysis.

However, I find mixed opinions on internet about a sensible threshold, which is expceted to be 1.1-1.5. Yet, I have seen publications that have used 0.15/0.25 logFC for the analysis.