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Questions related to Cloning
Why siRNA peak AUC vary for sense and antisense strands after denaturation. Isn't it ideally it should be in 1:1 ratio and hence AUC to be same?
04 February 2025 1,779 0 View
If i must choose some of them, on any basis?
29 January 2025 8,628 2 View
What Matrices are to pay first priorities?
29 January 2025 7,175 3 View
We are testing the effect of inhibition of proteasome on the protein level of p27 in diseased cells Surprisingly, the protein level of p27 not only is not accumulated but is even lower than in...
28 January 2025 7,536 0 View
Hello everyone, I’m working on a dataset of metabolic data collected during a 48-hour experiment. The data was recorded every 2 minutes and 30 seconds, resulting in a large number of time points....
28 January 2025 1,366 1 View
Hi, I am having trouble with selecting an ideal control for my antisense oligo cell proliferation experiment. I have some candidate controls, and am trying to compare their effect with H2O + RNA...
24 January 2025 2,540 0 View
If you are involved in school administration or as teaching faculty, what does concerning behaviour of a student mean to you?
23 January 2025 1,870 5 View
Hi all, I want to introduce two separate receptors in human T cells. I have the receptors in separate retroviruses and plan to follow a retronectin-spinoculation protocol. I was wondering if...
21 January 2025 8,177 4 View
I have data (attached) consisting of students' scores under the following categories: Activists, Reflectors, Theorists, and Pragmatist. I am to complete the data under the following: a. Grading...
20 January 2025 6,868 4 View
Hello, These rice shaped minerals were found on some of my Upper Triassic slides from Italy. If anyone has any idea what they might be or how they got there, I haven't changed my slide...
20 January 2025 7,754 6 View
Hi! I have been growing porcine primary intestinal cell culture for the past week and I noticed these things growing at the bottom of my flask. I wanted to ask if anyone has any idea what it may...
18 January 2025 2,690 2 View
Hi everyone, I’m following a protocol to flush bone marrow from mouse femurs and tibias, but I consistently get little to zero cell yield. After RBC lysis and centrifugation, I don’t see a white...
18 January 2025 6,741 5 View
Format: (Vancouver reference style) Author(s). Title of thesis [type of thesis]. Place of publication: Publisher; Year of publication. Example: Taylor R. The impact of climate change on...
18 January 2025 397 0 View
The discussion on how our experiences influence the understanding and application of scientific ethics during doctoral training is fundamental to various aspects of researchers' academic and...
17 January 2025 5,038 1 View
Regarding the selection of commercial virus plasmids for constructing immortalized porcine alveolar macrophages, how should one choose between commercial lentiviral vectors and retroviral vectors...
13 January 2025 7,124 0 View
I often do post-acquisition on mass spectra to increase mass accuracy for untargeted lipidomics or top-down proteomics studies. The procedure helped me with the Waters, Sciex, and Bruker mass...
11 January 2025 8,097 4 View
whenever I try to optimize the geometry of the molecule in anion it shows #2070 error
10 January 2025 3,121 1 View
I have used Plasmid DNA with a specific insert cloned in the Plasmid as a template for setting up the IVT reaction. Here, I have used both T3 and T7 RNA polymerases to synthesize the antisense and...
07 January 2025 3,901 3 View
I want to make a repoet for a code of one paper, So i want to use "app.korbit.ai/" but i couldnt understand HOW!!! If you know some information about it, please let me know. Thankyou
10 December 2024 7,115 0 View
"Can someone help me download the following two articles? I couldn't find them on Google Scholar or SCI-Hub. I want to find the exact formulations of MMN media and Pachelwski media." The...
09 December 2024 4,823 2 View
dificultades en la conceptualización de conceptos químicos
05 December 2024 3,693 4 View
Hello! I performed an SDS-PAGE with 500 ng of Spike protein (under denaturing conditions) using a 10% gel. In the Spike lane, I observed a band at approximately 180 kDa and another at 120 kDa...
03 December 2024 2,003 2 View
I forget to add mercaptoethanol to my SDS sample buffer of my protein sample. I already heated my sample and stored it. What should I do ?
23 November 2024 1,035 1 View
I want a research proposal using that research question
21 November 2024 4,400 1 View