What kind of software are you using to analyze courtship behavior in Drosophila?

Zhang Binglong @Zhang-Binglong

23 December 2023 0 7K Report

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Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

05 August 2024 8,836 2 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

The complex interactions between oral microbiota and oral mucosal immunoregulation: implications for oral health and disease development?

This research question explores the complex interactions between oral microbes and oral mucosal immune regulation. The diversity and function of oral microbes have important impacts on oral health...

09 July 2024 7,883 0 View

How to solve the problem that the film obtained by magnetron sputtering on the ternary alloy target A2BC is ABC (not A2BC)?

Using magnetron sputtering with an alloy target composed of A:B:C in a 2:1:1 ratio, the resulting thin film composition is A:B:C=1:1:1. How can you achieve a A:B:C=2:1:1 composition? What are some...

24 June 2024 1,605 0 View

A small question about the principle of lasers…?

Given the energy level diagram of a ruby laser shown in the right figure, where \( R_{02} \) is the pumping rate, and \( \tau_2 \) and \( \tau_1 \) are the lifetimes of levels \( n_2 \) and \( n_1...

23 June 2024 4,855 0 View

Can the matter wave theory which suggests that when matter waves resonate be used to solve HTS ?

Can the matter wave theory, which suggests that when matter waves resonate, the energy input from the outside is minimal, be used to solve all superconductivity problems? The matter wave theory,...

17 June 2024 7,957 0 View

There is any relationship between critical temperature of superconductor and the atomic weight & radius?

till now, there are many superconductors. Is there any relationship between critical temperature of superconductor and the atomic weight & radius? Now the isotope effect is one relationship...

12 June 2024 1,908 3 View

How to promote an academic journal and get high-quality manuscripts?

International Journal of Complexity in Applied Science and Technology(https://www.inderscience.com/jhome.php?jcode=ijcast) is a new journal. Can anyone tell me how to promote the new journal and...

09 June 2024 1,922 1 View

DNA FISH with multiple signal spot ?

I am doing DNA FISH on mice stem cells to detect an endogenous gene location. The expected result should have two signal spots, representing the two copies of the allele. However, what I see is...

06 June 2024 941 1 View

How to do CUT and TAG normalisation for EZH2 inhibited cells in H3K27me3 pulldown samples?

Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...

21 April 2024 3,197 0 View

Problem with Mirdeep2 and Randfold output?

Hello team, I am using Mirdeep2 to identify miRNA in my organism (none model organism) which has no previously identified micro_RNAs. My organism is genetically close to Drosophila melanogaster....

14 April 2024 9,090 0 View

Which HA beads are the best for Co-IP (drosophila tissue)?

Hi all, I am using HA beads to do a Co-IP (using drosophila whole body), then do mass spec to detect the interacting partners that pulled down by HA. The one that I tried before was not good, it...

09 April 2024 2,374 0 View

What online resources can I use to obtain the gene sequence of a widely used modified transgenic protein (QF2w from N. crassa)?

Hello, Apologies if there's an obvious answer to this, I'm a first year PhD student and this task is entirely new to me. I'm including a lot of detail to convey my question clearly. In D....

04 April 2024 9,635 3 View

Apart from using red light as a source to mimic DD conditions to Drosophila melanogaster to study the behaviour can any other light source be used ?

behavioural study of Drosophila melanogaster in DD conditions

08 February 2024 2,608 0 View

Can i get a specific protocol or buffer to extract protein from drosophila body. I even need a procedure for SDS-PAGE specifically for this organism?

I need a detailed protocol of SDS-PAGE only for drosophila if anyone has done before

16 November 2023 7,784 1 View

When using the UAS-GAL4 system in drosophila, does incubation of white prepupa at 18C reduce the efficiency of the driver ?

When using the UAS-GAL4 system to express the transgene in a fly (at the white prepupa stage), does altering the temperature from 25C to 18C reduce the effciency of the GAL4 driver in turn leading...

15 October 2023 5,416 4 View

I am unable to amplify my 3.8kb gene from cDNA of Drosophila?

The Tm of primers is 65 degrees and I am getting only non specific bands around 1kb. I amplified using Q5-HF Polymerase. How can I improve the specificity to get my desired band?

17 September 2023 4,956 5 View

How to use a clustering approach like degPatterns() with only a results_apeglm output?

I have an unusual question: I am working on a Erasmus internship project with Drosophila mutants at 2 different timepoints and with WT, KO and KI condition. A company analyzed the data using...

11 September 2023 1,743 1 View

How can I characterize the dark spots (probably melanocytic tumors) in drosophila melanogaster?

Hello Everyone, I'm a master's student and I'm currently working with drosophila melanogaster mutants. Right now I have a mutant that has dark spots in various regions of the fly's body (in the...

03 September 2023 7,795 0 View