Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

No title

23 February 2021 4,014 3 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to find a particular aptamer complementary sequence?

I can find several papers that give me a guide for choosing a suitable aptamer sequence 1. But how to find a property aptamer sequence 2(partially complementary to sequence 1)?

09 February 2021 3,511 1 View

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam?

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam? Could you provide some MATLAB code to me , a beginner for GA. Thank...

03 February 2021 3,789 5 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

CRISPR/Cas9 oligo design ideas and suggestions?

I need to design new oligonucleotides that can be used for gRNa/Cas9. The oligonucleotides need to target a gene from melangoster drosophila. Where do I start? How to find the gene to knock...

15 December 2020 4,729 3 View

How does the Laser Power Meter measure the light? Is it the power of light per unit area?

I am doing optogenetics on Drosophila larvae. I need to use the Blue light and I have to measure the light intensity. For this purpose, I am using "Sanwa Laser Power Meter LP1". The display shows...

11 August 2020 8,696 3 View

Does using ethanol for de-waxing drosophila heads before dissection interfere with future protein extraction or fly brain imaging?

I have heard that dipping fly heads in 70% ethanol before placing in cold PBS for dissection can help strip the heads of wax and allow them to stay submerged in the PBS during dissection. I was...

10 August 2020 4,373 2 View

Protocol for combining FISH with IHC?

Hello, we are developing a protocol for combining FISH and IHC on Drosophila embryonic brain, using FISH to localize mRNA and IHC (2 antibodies) to mark neuropil and neural tracks. We thought...

02 March 2020 3,896 1 View

Protocol for combining FISH with IHC?

Hello, we are developing a protocol for combining FISH and IHC on Drosophila embryonic brain, using FISH to localize mRNA and IHC (2 antibodies) to mark neuropil and neural tracks. We thought...

29 February 2020 3,291 3 View

What chemical or substance can I use as an alternative to propionic acid for breeding Drosophila?

Hello. I am looking for a cheaper alternative to propionic acid which I can use for breeding Drosophila. I am currently teaching Evolutionary Biology and I would like my students to conduct...

11 December 2019 6,313 2 View

Another solvent different to DMSO and Ethanol for picrotoxin?

Im working with drosophila, and i want to avoid those solvent because the influence the may have in memory.

11 December 2019 3,638 2 View

Best way to quantify tumor size/area from confocal images?

I have been imaging many z stacks of Drosophila mid/hindgut tumors using a leica confocal microscope. I'm wondering what the best way to quantify these tumors would be. They are GFP+. I know both...

11 December 2019 7,260 3 View

Best way to quantify tumor size/area from confocal images?

I have been imaging many z stacks of Drosophila mid/hindgut tumors using a leica confocal microscope. I'm wondering what the best way to quantify these tumors would be. They are GFP+. I know both...

02 December 2019 4,584 3 View