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Questions related to Cloning
Research context: Our feline case study (DOI: 10.13140/RG.2.2.19040.19200) identified an interesting correlation between iris coloration and genetic disease status that could potentially serve as...
07 June 2025 5,965 2 View
que grupos vulnerables en situacion de riesgo existen en Ecuador
05 June 2025 7,359 1 View
Hello, I tried to prepare some samples for scanning electron microscopy (SEM) of viruses. To ensure the presence of viruses on the slides. I have placed a drop of 50 ul of suspensionion titrated...
29 May 2025 1,379 3 View
How can I add a category of research items (for ex. Books)? Thanks lorenzo gagliardi
26 May 2025 3,690 2 View
I need Tissue sample which will be preserved for DNA and RNA Extraction. Need to know is there any commercially available transport media ?
24 May 2025 5,778 4 View
Docking result analysis using discovery software
24 May 2025 2,627 3 View
Two weeks ago, I run some PCRs, sometimes I cannot get amplification but other times I indeed get it. But now that I am trying to replicate it is not possible, that could not be unspecific bands...
22 May 2025 3,537 3 View
I have tried to work on antagonistic activity of Bacteria with Coffee berry disease (Colletotrichum kahawae grown on PDA and also Nutrient agar with 10%glucose. Am I correct? I am waiting for the...
19 May 2025 9,255 2 View
If we measure the concentration of nitrate in a single leaf at different times of a day (at early morning, daytime, and evening times), the concentration will show a difference. Why?
18 May 2025 5,958 2 View
Am conducting a research about assessment of open defecation I have already collected and some other preliminary information.Some guides on the best software to use in analyzing the data and the...
15 May 2025 7,899 1 View
I’m working with a recombinant P450 enzyme that I’ve directed to the periplasmic space using a signal peptide. Since I will use this enzyme for downstream applications, maintaining its activity is...
13 May 2025 6,241 3 View
As far as I have learned, after the necessary pre-processing, I prepare my protein and ligand for molecular docking with AutoDockTools with the following workflow. For protein preparation,...
12 May 2025 7,087 4 View
I am currently working on generating a knockout of the IIIG9 gene in AF22 cells using a lentiviral vector containing a puromycin resistance cassette. As part of the preparatory work, I attempted a...
11 May 2025 5,360 0 View
Hello, I would like to inquire whether enriching the Tryptic Soy Broth (TSB) medium with supplements such as yeast extract, meat peptone, amino acids, and vitamins can enhance the growth rate of...
05 May 2025 7,865 4 View
I am currently working on evaluating the antifungal activity of the cell-free supernatant from certain probiotic strains. For this, I need to use a positive control plate. In our laboratory, we...
05 May 2025 6,218 3 View
If so, how to determine the most suitable model? Like PFO R2 0.998 PSO R2 0.995
05 May 2025 9,827 5 View
I'm a Master's Degree student currently studying plant pathology. I'm changing course from studying plant breeding to plant pathology. So, it's a new area for me and I'm lacking many. I chose this...
03 May 2025 3,641 2 View
Hello everyone I am transfecting my primary endothelial cells using Lipofectamine LTX. As controls, besides other controls such as non-transfected cells and the transfected cells with empty...
02 May 2025 8,570 1 View
Does anyone have a protocol or a reference for the extraction of RNA from ovine whole blood samples? I plan to run RT-qPCR following extraction. I have been looking for some detailed references...
01 May 2025 9,002 1 View
This question explores whether meme marketing leads to increased sales and brand recognition in Pakistan. It examines how memes influence consumer behavior, especially among younger audiences, and...
29 April 2025 506 3 View
How can I use kinetic models to apply to complex chemical reactions?
26 April 2025 5,362 6 View
Can anyone tell how can I predict the secondary structure of tmRNA of a specific species of bacteria(Leptospira interrogans) ?
19 April 2025 5,137 1 View
In the context of rapidly evolving digital media ecosystems, how can traditional theoretical frameworks in Mass Communication such as Agenda-Setting or Uses and Gratifications, be adapted to...
19 April 2025 2,686 2 View
Hello, I've been recently doing qPCRs to compare how a certain stimulation can increase the gene expression for a particular trannscription factor. I have some issues as this technique is not...
16 April 2025 8,532 3 View