Hi colleagues! So, my friend at the lab is trying to get karyotype from Amnion fluid, can anyone with experience help us find the issue with this?

07 February 2024 1 4K Report

He gets good culture and all cells look good and meet the requirement needed and explained in the protocol. But at the end, he can not get a good chromosome spread. We will really appreciate if someone could share their experience with us.

Sabine Strehl

Have a look at this thread on RG:

https://www.researchgate.net/post/What_is_the_best_technique_for_chromosome_spreading

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