What is cause of qPCR curves to be sharp?

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Can Kiessling

Can you also show the same image in log for RFU as well as melt curve?

What is the sample type, FFPE, synthetic DNA etc?

Philippe Paget-Bailly

Hello,

if you can, try to change the baseline cutoff (at which cycle your apparatus start to take into account fluorescence, i'm not talking about the threshold).

Sometimes those weird sigmoid curves are just a software problem.

Hope it helps,

Philippe.