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Questions related to Cancer Stem Cells
I am working on colon cancer stem cells to see the impact of combinational therapy.
24 December 2020 10,192 3 View
in Gastric cancer both cancer stem cells and tumor microenvironment prevents the action of therapeutics and drugs. a. How can I find a co-relation between them? b. How can i evaluate their role...
07 May 2020 6,700 7 View
I would like to visualize cancer stem cells in breast cancer tissues, isolated from patients, using Hoechst 33342 stain. If anyone can provide a link to the protocol, it would be very helpful.
13 March 2020 4,516 2 View
I am working on CD133+ cancer stem cells from B16F10 cell line. I use serum-free high glukose DMEM medium and also add EGF, EGF as growth factor. But CD133+ cells died after 3 days. Do you have a...
01 January 2020 6,223 3 View
Sphere formation is known among normal or cancer stem cells. But whats the underlying mechanism of cells to come together and make sphere?
06 June 2019 9,624 4 View
Hello everyone, I would like to know about the formation of spheroids (for cancer stem cells) in cancer cell lines using B27 supplement. If someone has succeeded in developing spheres in cancer...
30 May 2019 3,296 3 View
In my quantification of genes expression between cancer cells and cancer stem cells one of the gene has lower Expression in cancer stem cells than cancer cells. What is the significance of this...
05 May 2019 6,643 2 View
We are isolating single cell suspension from breast tumor tissues by enzymatic digestion (trypsin digestion). But I am getting low cell count after passing the suspension through 40 micron filter,...
27 March 2019 5,743 3 View
Hello, We are currently trying to produce neuerosphere cultures from parental glioma cells lines. Parental cells were disassociated and cultured in neurobasalA serum free media containing...
07 March 2019 9,043 2 View
03 March 2019 7,360 4 View
03 March 2019 2,875 2 View
I am working on treatment of gold nanoparticles (AuNPs) and gold nanocages on various cell lines (MSCs, Cancer cells, Cancer stem cells) I found out that the viability of the cells were decreased...
25 February 2019 3,960 3 View
02 February 2019 7,552 3 View
I have some rectal cancer stem cells that I started in NTCT p100s - they were doing great and finally started expanding quickly so I moved them to a NTCT flask (after Tryp-LE dissociation at 37C...
01 January 2019 7,124 4 View
I am working on induction of cancer stem cell like properties in MCF 7 and MDA-MB-231 cells. Is CD133 a good marker for identifying breast cancer stem cell or there are other markers? Can...
10 October 2018 9,070 0 View
Oxidative Stress and Reprogramming of Mitochondrial Function and Dynamics as Targets to Modulate Cancer Cell Behavior and Chemoresistance Open Special Issue on Hindawi's Oxidative Medicine and...
10 October 2018 7,511 0 View
Hi everyone! For my next Master's assignment, I am supposed to write a mini-review on cancer stem cells, more specifically, a comparison between cancer and normal stem cells and how to target...
08 August 2018 8,349 3 View
01 August 2018 1,200 2 View
I am asking what is the specific criteria that makes stem cells survive the serum free medium (supplemented with B27, N2, FGF and EGF) and the non-stem cells die?
06 June 2018 7,512 4 View
Tumor sphere formation have been used lately to isolate cancer stem cells and proliferate them. why the non-stem cells don't survive these conditions?
06 June 2018 1,299 5 View
Hello Everyone, I need some information on sphere and spheroid culture. I am trying to select cancer stem cells from ovarian cancer cell line OVCAR_8 and OVSAHO. I'm using serum-free DMEM / F12...
05 May 2018 2,412 0 View
hello, Is there anyone who has sorted MCF-7 using CD24-/ CD44++/CD326++(epcam) markers. I got articles with CD24/44. Please share some articles with FACS profile from mcf-7 and MDAMB 231 with...
05 May 2018 2,550 0 View
In cancer stem cells it is always mentioned that they have stemness properties and self-renewal ability. Are those the same thing but different names or there is a difference?
05 May 2018 9,829 4 View
Given that multiple myeloma is a monoclonal neoplasm which is originated from plasma cells, there is a controversy whether there exist cancer stem-like cells in MM or not. Could you give me your...
04 April 2018 4,504 0 View