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Questions related to Cancer Stem Cells
Hi everyone, I would like to identify ovarian cancer stem cells using ALDH+CD133+ signature. In the literature groups have used antibodies against either CD133/1 or CD133/2. Is there any...
06 May 2016 8,565 2 View
05 May 2016 171 2 View
I am trying to preserve a population of glioblastoma cells that I believe to be cancer stem cell. I wish to passage the cells and preserve their "stemness" during each passage. I need the right...
05 May 2016 1,696 2 View
I want to characterize the mammospheres using conventional PCR. I have performed the experiment 3 times but every time I have a problem with the results. I need a detailed protocol for the steps...
05 May 2016 8,778 1 View
myeloma stem cell isolation sorting culture
05 May 2016 225 1 View
01 May 2016 7,566 1 View
Can we use just normal flasks that are routinely used for cell cultures? I realized that it is the best if we don't let cells to attach the surface when culturing stem cells, especially breast...
04 April 2016 6,711 2 View
Can anyone suggest me the advanced books or articles discussing the similarities and differences between embryonic and cancer stem cells and highlights on the research development and applications...
04 April 2016 7,158 1 View
Cancer cells and cancer stem cells undergoing invasion, EMT and migration exhibit different metabolic states. Does cellular energetics change during these processes?
04 April 2016 9,835 3 View
As GBM cancer stem cell marker ?
04 April 2016 5,110 2 View
02 April 2016 4,196 2 View
Among the unfortunately (too) large set of chemoresistance mechanisms in cancer cells is the so "popular" ABC-related pump / MDR phenotype. While there are hundreds (if not housands) of articles...
03 March 2016 9,678 13 View
20 hours? 24 hours?
09 February 2016 8,974 2 View
I am using gefitinib to inhibit the EGFR. I want to perform RT qPCR on EGFR target genes to make sure the drug is actually working.
08 February 2016 2,537 2 View
Is there any evidence of that?
04 February 2016 4,689 4 View
02 February 2016 1,112 5 View
The main ‘party trick’ of cancer stem cell (CSC) seem to be that it provide another explanation for tumour heterogeneity (i.e. cell divide asymmetrically give rise to progeny cells that are...
02 February 2016 2,111 11 View
-Monitoring of therapy -prevention of recurrence
02 February 2016 2,004 4 View
02 February 2016 7,946 3 View
02 February 2016 9,178 2 View
I have tested my glioblastoma U87 cancer cell lines for cancer stem cell markers. I wish to see a way other than stem cell marker expression, the potency of my cells.
19 January 2016 5,275 3 View
I have obtained some glioblastoma cancer stem cells and I want to determine their self-renewal potency based on the colony forming ability. If there is any other assays to determine potency for...
16 January 2016 504 2 View
01 January 2016 7,028 3 View
Hello everyone, I am planning to assess the effect of a drug on cancer stem cells proliferation. Giving that the mammosphere assay is an efficient way to assess cancer stem cells proliferation, I...
01 January 2016 9,463 1 View