Contact experts in Cancer Stem Cells to get answers
1,571 views 307 posts
Questions related to Cancer Stem Cells
The event of death by metastasis or recurrence is very common, many researchers have linked the tendency of some tumors to re-appear to the presence of occult or dormant cancer cells that may have...
01 January 2016 5,445 4 View
I have obtained some glioblastoma cancer stem cells and I want to determine their self-renewal potency based on the colony forming ability. If there is any other assays to determine potency for...
01 January 2016 9,486 2 View
dear everyone, How to analyze mammosphere cell death using the PI staining. I am planning to use a FACS analysis but I am affraid to get false results after forcing cells constituting mammospheres...
12 December 2015 5,442 4 View
On of surprising effect of glucocorticosteroids is increase expression of eNOS in endothelial cells, elevated expression of eNOS is associated with aggressiveness in glioma and it’s also required...
12 December 2015 2,708 3 View
hepatic cancer stem cell, interferon signaling STAT, PKR Is there any molecular linkage between interferon signaling and hepatic cancer stem cells?
12 December 2015 9,540 2 View
I've come across the term twice and was wondering what the term means. Does it concern a degree of 'stemness' these cells have acquired, or a late stage of autophagy/apoptosis? I'm very curious...
25 November 2015 7,382 2 View
Anyone knows about isolation and enrichment breast cancer stem cell by culturing MCF-7 in the low attachment surface? In the process to form into a tumorsphere, how often do you add new medium?...
11 November 2015 4,271 3 View
11 November 2015 4,911 2 View
Just wondering.
11 November 2015 7,890 3 View
We have normal in vitro facilities to culture the normal cancer cells. I want to work on cancer stem cells. Could you please suggest me what are the effective methods to maintain the cancer stem...
20 October 2015 8,585 4 View
10 October 2015 5,453 5 View
I want to isolate the cancer stem cells from cancer cell lines. But most of the people are isolating the cancer stem cells by using FACS. But in my lab it is not available. So I want to use...
10 October 2015 8,569 5 View
I am trying to understand whether cancer stem cells are the metastatic cells in cancer progression because of their ability to self-renew and proliferate on a single cell level.
10 October 2015 4,393 0 View
both mammosphere and foci have character of forming in sphere shape? so we can differentiate them?
09 September 2015 6,018 2 View
I am working on induction of cancer stem cell like properties in MCF 7 cells. Can anybody help me with suggesting which would be a suitable marker for this identification of breast cancer stem...
09 September 2015 4,302 10 View
I have sequenced tens of single cells of bladder cancer, and got the gene expression profile of each single cell. I am trying to speculate the cancer stem cells mixed in these cells using...
10 August 2015 9,251 5 View
The oncolytic viruses are known to target the cancer stem cells along with the cancer cells. 1. How effecient are these oncolytic virus in targetting cancer stem cells 2. Whether the...
08 August 2015 9,679 3 View
I need to analyses some pathways connected with cancer stem cell related genes. Waiting for your answers. Thank you
08 August 2015 814 4 View
08 August 2015 2,233 6 View
I am trying to harvest cell from matrigel coated flask plaese guide me I have tried trypsin but didn't work . I don't have matrigel dissociation medium
08 August 2015 6,405 5 View
During preliminary exposure of these cells to FGF, EGF, B27, and insulin factors; only a small amount of cancer stem cells was detected by flow cytometry analysis (CD133, CD44 positive cells)...
08 August 2015 6,798 1 View
The cells were flow sorted and was grow in DMEM F12 media without serum and supplemented with hEGF, hFGF and 0.4% BSA
08 August 2015 6,719 6 View
I am culturing cancer stem cells and have a question to the composition of the media. After isolation of a patient with NSCLC and enrichment of the CSCs we now culture them under free-floating...
07 July 2015 3,557 2 View
Which colorectal cancer cell lines are easily transformable using lipofectamine apart from HCT-116.
07 July 2015 7,048 3 View