I would like to visualize cancer stem cells in breast cancer tissues, isolated from patients, using Hoechst 33342 stain. If anyone can provide a link to the protocol, it would be very helpful.
https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf for immunohistochemistry protocol.
https://www.thermofisher.com/order/catalog/product/62249#/62249 for Hoechst 33342 dye
Hello
Kindly look at link below :
https://www.bosterbio.com/protocol-and-troubleshooting/immunohistochemistry-ihc-principle
Good Luck
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