How to prepare an algal sample for FT-IR spectroscopy?

Can we measure the bulk composition of biomolecules present within an algal sample directly by placing its powdered form on a FT-IR spectroscopy stage? Or does it require a particular kind of...

30 January 2021 2,303 4 View

How to polish the samples which are very prone to oxidation for metallography?

I am trying to polish the rare earth based sample for SEM analysis. But the sample is getting oxidized immediately . Can anyone suggest a way to polish these kind of samples?

27 January 2021 4,699 2 View

How can I compute the distribution of dipole moment as a function of the angles between the dipole vector and solid surface through Lammps command?

I want to plot the distribution curve of dipole moment as a function of angles between dipole vector and solid surface in the presence of external electric field. My system is consisting a...

17 January 2021 1,508 4 View

How do we solve optimization problems involving a maxima or minima in the objective function ?

I am trying to solve a multi-objective optimization problem using the Pyomo library in Python. The objective function includes operational optimization of an electrolyzer for hydrogen production...

11 January 2021 8,018 3 View

How to dissolve PVDF in DMAc solvent?

I need to dissolve 1.5gm of PVDF tablets (from Sigma ALdrich) in 25gm of n-n DiMethyl Acetamide (DMAc) solvent. I have stirred the solution for 2 hours using magnetic stirrer and it did not seem...

02 December 2020 1,777 3 View

Any researchers working on Mucuna pruriens with respect to tissue culture?

15 November 2020 8,675 1 View

Hello to all the eminent researchers. I need an insight of how to analyze the performance of PEM cell using machine learning?

I have a PEM cell dataset, and I want to plot the degradation curve or polarization curve using machine learning algorithms. How can I approach in this regard. I have to design an algorithm to do...

18 October 2020 7,909 2 View

Has chemistry Nobel Prize really become the biology prize ??

07 October 2020 8,119 6 View

How can I confirm that a gene is downregulated in cancer cells through PCR?

A particular gene (gene A) is reported to be downregulated in cancer cells as compared to the normal cells. How can you prove this with the help of PCR?

27 September 2020 5,292 4 View

Why my absorbance values are in negative for antioxidant assay by dpph method ?

plant extract stock is 10 mg/mL from which I pipetted 20, 40, 60, 80, 100, 200 uL and made up to 3 mL using methanol then i added 1 ml of 0.004% dpph solution (dissolved in methanol) incubated in...

02 September 2020 1,218 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View