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Questions related to Cancer Stem Cells
malignant cell is formed according to the rule of :initiation,promotion then progression ,is the stem cell follows the same rule? OR/ it follows another theory ?
25 May 2017 8,633 3 View
Is EMT cancer-type-specific? Or does EMT occur in all kinds of tumor? I'm curious how specific the EMT signature is among different types of cancer. Is there any cancer type that EMT prefer? Thx
23 May 2017 4,889 3 View
Hi What does the different shape of cells mean, invivo subcuatneous, cells are from single sorting spindle shape and round shape of pancreatic cancer cell line from KPC mouse ? thanks
21 May 2017 3,061 1 View
05 May 2017 5,632 1 View
05 May 2017 2,399 4 View
05 May 2017 7,220 3 View
Hello, I have isolated cancer stem cells from MCF-7 cells. I want to characterize these cells. Is there any option available other than FACS? Can we do it with PCR or western blot for markers of CSCs?
04 April 2017 500 5 View
Hello, I wonder if we want to study a gene silencing in Cancer stem cells and we are doing a Knockdown in cancer cells. After a stable knockdown if we isolate cancer stem cells from same cell...
14 March 2017 3,537 2 View
I am aware that migration/wound healing, sphere formation, flow cytometry, and immunoblot are all good experiments for these types of questions. I'm not sure if these experiments answer the EMT or...
03 March 2017 4,812 3 View
Dear all, I genetically engineered the human primary cells with CRIPSR-Cas9 system and currently can passage them stably in vitro. The NGS analysis showed multiple mutations of target sites but...
03 March 2017 8,053 3 View
03 March 2017 2,074 2 View
Does anyone know the positive control cells for stem cell marker CD133.
03 March 2017 5,781 0 View
Hello, I am working with breast cancer. I have read many articles in which they have isolated cells from cell lines. As the number of cancer stem cells is very less so is it possible to increase...
03 March 2017 3,869 10 View
Hi all, I have got a six months research fellowship. I am in search of some relevant labs to work in. My PhD project aims at the identification and characterization of novel altered proteins in...
03 March 2017 10,181 2 View
does anyone know that overexpression of ido1/2 or TDO2 in cancer cell would change the cellular Secretomics? any scientific reports?
03 March 2017 7,438 2 View
I have knockdown shRNA (TRIPZ) cell lines, that I now want to "knock in" a gene that I would tag with GFP, and transfect with lentivirus. Does anyone have any experience with this, they could...
02 February 2017 8,906 1 View
I want to assess the effect of radiation on normal human astrocytes, which are co-cultured with glioblastoma cells. However, a clonogenic assay is not suitable for this since astrocytes do not...
02 February 2017 3,921 7 View
Hi, I have a question, if I want to cultivate a549 (cell of lung cancer) and I want to separate cancer stem cells (cd133+) from a549 and I want to make a comparation between cd133+ and cd133-...
01 January 2017 6,703 4 View
I have reported that chemotherapy for the patient with Li-Fraumeni syndrome results in the ectopic CD44 variant-positive cancer stem cells. Given that chemotherapy often increases the ROS level,...
01 January 2017 6,346 3 View
cancer
01 January 2017 5,435 0 View
Neurotransmitters have been shown to drive tumor progression in the prostate glands and the stomach. In particular, it has recently been shown that acetylcholine promotes proliferation of...
01 January 2017 1,254 5 View
Hello all, I'm looking to start using an ALDH assay as a marker of stem-like characteristics in Cancer Stem Cells (CSCs). These assays are quite expensive and before I commit to purchasing one, I...
21 December 2016 9,120 4 View
A recent report in Nature (Dec. 15, 2016) has identified 17 unique gene expression signatures for AML in leukemic stem cells, which could be useful in predicting “targeted therapies” and has the...
12 December 2016 7,805 2 View
12 December 2016 4,406 5 View