Hello Everyone, I need some information on sphere and spheroid culture. I am trying to select cancer stem cells from ovarian cancer cell line OVCAR_8 and OVSAHO. I'm using serum-free DMEM / F12 medium with EGF / FGF / heparin and B27, in Corning P24-ULA plate.

Can I call the resulting "sphere" as spheroid or ovosphere (as mamosphere)? There is a difference between 3D culture of spheroids and sphere? Thank you for your help!

Audrey.

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