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Questions related to Cancer Stem Cells
I want to know about the advanced theraphys against cancer stem cells please anyone of you tell me about these
12 December 2016 3,466 0 View
Ideally, a further breakdown by cell type (somatic vs stem cells) and the outer cell surface would be nice.
12 December 2016 470 4 View
Epithelial Mesenchymal Transition
11 November 2016 4,908 2 View
I try to do the sphere forming assay with oral squamous cell carcinoma cells to test the effect of a drug against cancer stem cells.
10 October 2016 10,095 0 View
For cancer cells can Iuse the technical variation (array- hybridization)for CNV?
10 October 2016 5,161 4 View
I am trying to insert a 60 bp shRNA into the pLKO.3G plasmid (variant of pLKO.1), yet nothing is working. I digest with EcoRI and PacI, anneal the oligos at 95 for 4 mins then 70 for 10 mins, then...
09 September 2016 1,704 5 View
Hi, i want to use 20ng/ml BFGF in culture media. How can i dilute 35 microgram bFGF/35 microliter Tris 5mM without any problem for breast cancer stem cells?
09 September 2016 2,108 1 View
Dear all, Currently I tried to co-culture some cancer stem cells accompanied with Peripheral blood mononuclear cells. As you know cancer stem cells` medium is serum free and we also know that...
30 August 2016 9,046 3 View
Which depression model for rats do you prefer? What is the best way to induce depression in rats? We would like to start some projects in this field and we do not have any experience....
26 August 2016 3,768 3 View
I came across a sound number of techniques regarding analytical approach on cancer stem cells or cancer initiating cells (CSC). But I could not able to find out any standardized method to increase...
15 August 2016 1,385 6 View
Carcinoembryonic Antigen (CEA) gene family contains 33 genes/pseudogenes clustered within a region of 1.5 Mb on chromosome 19q13.2. Apart from being a reliable tumor biomarker for colorectal...
08 August 2016 8,715 1 View
08 August 2016 9,975 6 View
08 August 2016 5,298 4 View
Hello. I have a problem with getting sphere from the AGS cell line. I want to get sphere from this cell line to receive cancer stem cells but its really hard to get sphere through low attachment...
31 July 2016 6,516 3 View
07 July 2016 6,318 3 View
Consider this scenario, where 35 year old female is diagnosed for breast cancer (malignant), her Breast tumor is completely dissected from her breast or body via mastectomy or lumpectomy. Later...
28 June 2016 1,038 2 View
I know there are three isoforms: CD133/1, CD133/2, and CD133/3 and also the fact that CD133/2 is the isoform that is used for hematopoietic stem cell isolation. Thank you so much in advance.
18 June 2016 4,446 2 View
We all know that CSC (Cancer stem cell) are germ line of tumor giving rise to body of tumor cell. Then why this body of tumor cell fails to carry single gene mutation or single gene alteration,...
06 June 2016 9,200 3 View
06 June 2016 1,906 6 View
MDA-MB231 is used by most, as well as its variants made by Joan Massague's lab, but any others, especially in the Her2+ category? Thanks.
06 June 2016 9,548 3 View
Hi guys, I recently developed a HEK293 cell line with an expression reporter at the endogenous locus of a gene interacting with EMT-associated genes. To test the functionality of the reporter...
06 June 2016 7,042 2 View
06 June 2016 9,590 2 View
I need ALDH positive breast cancer stem cells for my reasearch work. Does any one can provide me the information that whether this cell line is available at ATCC or provided by other companies. I...
06 June 2016 8,211 4 View
02 June 2016 5,768 3 View