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Questions related to Cancer Stem Cells
I am working with MCF7 and 293T cells. Do I have to know the # of passages that they have passed? If yes, what is the maximum passage's number, in general to my cells?
05 May 2014 3,337 18 View
There are reports which support idea of cancer stem cells, a multipotent cells which show resistance to chemotherapy and help in regeneration of cancer cells. There are many question which come...
05 May 2014 8,544 4 View
Experiments to study the innate responses during pre-patent schistosome infection.
05 May 2014 4,850 0 View
I would like to do it with spheres but it may be not easy.
05 May 2014 7,117 0 View
I am trying to seed 3000 breast cancer cells in 96 well plate and 6 well plate. I count the cells on 4 corners of the hemocytometer(after diluting the sample 1:10) and then take the average. For...
05 May 2014 3,801 10 View
While maintaining cancer cell lines originated from cattle squamous cell carcinoma we are troubleshooting an unusual problem as we are getting some spheroid like structures in our flasks...
11 April 2014 8,365 3 View
04 April 2014 312 3 View
My project is based on isolating the cancer stem cells and studying them. After using CD44 and staining them in KGM media, FACS sorting was done. The sorted cells were found to die within 2 days....
04 April 2014 2,482 8 View
Some in vitro data using cancer cell lines has been published, which suggests reprogramming of non-tumorigenic cancer cells to cancer stem cells induced by ionizing radiation. Is there some...
04 April 2014 6,473 1 View
I'm planning on injecting some melanoma and hepatoma cell lines mixed with Matrigel into the mice subcutaneously. However, I'm not sure what the pros and cons of diluting Matrigel with HBSS, RPMI,...
03 March 2014 786 4 View
EMT generate cells with stem like properties. I was wondering whether it reverses the the differentiated cells into undifferentiated state? My cells undergo dedifferentiation and acquires cancer...
03 March 2014 3,489 4 View
I actually plan to do RNA isolation/cDNA/QRT-PCR from sorted hematopoietic stem cell fractions. Does anybody have experience in this? How can I downscale the number of cells used for RNA...
03 March 2014 6,033 8 View
I reached your paper but it is in Chinese so I could not understand it. Could you help me to understand how B27 and insulin affect MCF-7 derived mammospheres please? Thank you
03 March 2014 3,418 1 View
Spheroids or spheres derived from cancer cell lines usually consist of proliferating & un differentiated cells. Assuming this, what can be the alkaline phosphatase activity of cancer derived stem...
03 March 2014 6,172 1 View
Just like we can elucidate bacterial growth by calculus.
07 February 2014 2,088 4 View
Can an original culture of 5-10 cells grow to be as large as 10 to the power of 12 cancer cells replicates in just 20 hours? Could this be correct or may his methods have been translated wrongly?...
02 February 2014 5,251 3 View
02 February 2014 4,000 19 View
I am interested in understanding the role of an enzyme in cancer stem cell function and I over expressed this enzyme (stable transfection). My flow date ie., side population assays show good...
02 February 2014 9,942 4 View
I am going to seed cells (colon cancer cells) in a 24 well-plate and after 6-7 hour I want to count them to find out the seeding density.
02 February 2014 8,783 2 View
I am going to make a peptide for cancerous cell line treatment.
02 February 2014 5,303 8 View
Although the malignant tumors at the initial stage from the stemness starts as a Monoclonal origin, upon the further proliferation, a secondary clonal population of cancer cells are generated....
02 February 2014 5,741 1 View
Does cancer stem cell play a role in metastasis?
01 January 2014 9,843 4 View
Does anyone know of a published experiment of Side Population (SP) analysis using a dye other than PI or 7-AAD for dead cell exclusion?
01 January 2014 3,277 4 View
What are the possibilities of a Cancer Stem Cells targeted drugs to enter clinical trial? Consider that it might equally target the adult stem cells?
01 January 2014 5,630 5 View