What is the best choice for a ribosomal protein used as a loading control in WB?

Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...

05 June 2019 2,726 3 View

Has anyone used the SIRT1 Activity fluorometric Assay Kit from Abcam?

Hi everyone. I am considering buying the SIRT1 Activity fluorometric Assay Kit from Abcam (cat. no. ab156065). However, after reading very carefully the protocol booklet, I am quite dubious. What...

04 May 2018 1,662 4 View

Has anyone used DRP1 (Phospho-Ser616) antibody from Biorbyt?

Hi. I am currently studying how certain treatments can worsen cancer cell behaviour through the alteration of mitochondrial dynamics. Literature reports show that mitochondrial fission is...

04 May 2018 7,016 2 View

Open Special Issue on OMCL - Mitochondria as a Central Hub in the Cellular Response to Redox-Active Environmental Pollutants ?

Open Special Issue on Oxidative Medicine and Cellular Longevity Mitochondria control critical cellular functions, including redox homeostasis, apoptosis, and autophagy. Several pathologies arise...

31 December 2017 3,372 2 View

Uneffective treatments with resveratrol or SRT1720 for SIRT1activation. What's happening in my HUVECs?

Hi everyone. I 've just tested two classic SIRT1 activators (resveratrol and SRT1720) in my HUVECs. Both compounds were dissolved in DMSO, and the final concentration ranges were 0-20 microM (RV)...

11 December 2017 5,796 6 View

Non-specific antibody binding or detection of complexed/immature forms of GSTA2?

Hi everyone. I have just used for the first time a NovusBio GSTA2 antibody (cat. no. H0002939-D01P). As reported in the figure , three different samples were used in our preliminary Western...

09 October 2017 6,777 7 View

Does anyone know the rate of disappearance of H2O2 in an acqueous solution?

06 July 2016 4,464 7 View

Band choice in SIRT1-directed Western blotting?

04 May 2016 5,936 12 View

Possible non-parametric alternative to a three-way ANOVA. Any suggestion?

03 April 2016 3,859 19 View

Weird phenomenon: spontaneous Western blot revelation and brown band appearance. How could that be possible?

02 March 2016 7,620 13 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View